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mammalian cell culture - BTEC 20 (3 units)
mammalian cell culture - BTEC 20 (3 units)
Introduction to techniques for culturing and maintaining mammalian cells, including proper use of equipment, aseptic technique, media formulation, storage, counting and subculturing of cells. Contents include proper growth conditions, cell cycle regulation, cellular responses to DNA damage, growth patterns, viability assays, transfection and an introduction to the use of fluorescent molecules in visualization of cellular structures. BTEC 20 is a prerequisite for BTEC 21C - Stem Cell Technology.
Some of what we do in Mammalian Cell Culture:
Some of what we do in Mammalian Cell Culture:
Basic mammalian cell culture techniques such as: Media preparation, plating, subculturing, thawing, freezing and storage
Aseptic technique
Counting cells and calculating cell concentrations and densities
Analysis of cell growth and plating efficiency
DNA damage and survival assay
Design a cytotoxicity assay
Introduction to fluorescent cell markers and microscopy
Transfection
Schedule:
Schedule:
Offered Fall & Spring (Day & Evening/Weekend Sections) – 6wk course - MWF 9am-12pm or TTh 2-5pm and Sat 12:30-3:20pm
Check the current schedule here.
Pre-requisites: None. A basic understanding of biology (DNA, replication, transcription, translation) and chemistry is needed - recommended course preparation is Bio 11 and Chem 32.
Programs: BTEC 20 is a technical elective in the Biotechnology AS Degree and the Biotechnology and Biotech. Work Experience Certificates.
CCSF Biotechnology students performing cell viability assays in BTEC 20.
BTEC 20 student passaging mammalian cells.
BTEC 20 student viewing mammalian cells by phase microscopy.
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