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recombinant dna technology - BTEC 115 (5 units)
recombinant dna technology - BTEC 115 (5 units)
An in-depth coverage of recombinant DNA/genetic engineering concepts and principles with a strong emphasis on the laboratory procedures involved in DNA and RNA manipulation. Techniques include DNA and RNA purification, spectroscopy, restriction enzyme analysis, DNA cloning, bacterial transformation, PCR and real-time PCR, site directed mutagenesis, and DNA sequencing. General topics in DNA/RNA structure and synthesis, control of gene expression, and genomics. Computer activities include DNA sequence analysis and PCR primer design using software such as SnapGene Viewer, Benchling, and NCBI resources.
Some of what we do in Recombinant DNA Technology
Some of what we do in Recombinant DNA Technology
Purify genomic and plasmid DNA (mini and maxi preps).
Design and run restriction enzyme analyses
Design, run, and analyze PCR and real-time PCR
Design and build DNA constructs using standard cloning techniques and Gibson assembly.
Run, image & analyze 4-5 agarose gels
Analyze Sanger DNA sequencing results
Learn concepts in modern genomics including CRISPR, new DNA sequencing technologies, new cloning techniques.
Applications of recombinant DNA
Applications of recombinant DNA
Genotyping, cloning, mutation detection, sequencing, microarrays, forensics, paternity testing, and evolutionary biology.
Pre-requisites: None. A basic understanding of biology (DNA, replication, transcription, translation) and chemistry is needed - recommended course preparation is Bio 11 and Chem 32.
Programs: BTEC 115 is a major elective for the Biotechnology AS degree, a requirement for the Biotechnology Certificate, and an optional requirement for the Stem Cell Technology and the
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