ada alvarez munoz
ada alvarez munoz
Bioscience Internship: Fujimori Lab, UCSF
Internship Mentor: Dr. Gloria Ortiz
Poster: Validating NanoLuc Constructs Used to Study the Histone 3 Interactions with KDM5A and its PHD Domains
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Prior to entering the CCSF Biotechnology Program I completed a B.S. in Biochemistry and worked as a pharmacy technician and ECG technician. I knew I wanted to return to a lab, but I had no idea what I wanted or how I was going to get there. I learned about the program through my sisters. They knew I wanted to find a long-term career that would allow me to continue to learn and grow.
Through the CCSF Biotechnology program I have become more self-aware, learned many new concepts, and found motivation. It has been a stepping-stone for me to find a job related to the field I studied in college.
I interned at the Fujimori Lab at UCSF, which studies post-translational and post-transcriptional epigenetic modifications. My project was focused on validating recombinant protein constructs needed for a bioluminescence assay that would allow my internship mentor to identify regulators of post-transcriptional chromatin modifications. To do this I employed mammalian cell culture, transfection of DNA constructs, protein lysis, Western Blot, bacterial transformation, DNA plasmid purification and PCR primer design. I also utilized multiple protocols to separate out nuclear and cytoplasmic proteins from transfected cells to determine the localization of the recombinant proteins.
I’m excited to have been able to develop considerable experience with a variety of molecular biology techniques as well as gain conceptual knowledge about epigenetics, a field I knew nothing of prior to my internship. The research I was involved in not only has the potential to develop a greater understanding of gene regulation, but also identify potential therapies for cancer. Through this experience I have found a new interest that feeds my need for continual growth and learning and hopefully guides me to the beginning of my science career.
Ada Alvarez Munoz - Validating NanoLuc Constructs - Spring 2022 CCSF Bioscience Internship.pdf
Ada in the tissue culture room at UCSF
Ada starting a DNA extraction at her internship in the Fujimori Lab
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