Flow Cytometry

The benchtop flow cytometer is equipped with two spatially separated light sources: an air-cooled argon laser with an emission at 488 nm and a red diode laser (Visible Red Diode Laser) with an emission at 635 nm. The optical configuration allows the analysis of up to 6 parameters, 4 of which are fluorescence, providing maximum flexibility in the choice of fluorochromes.

Specifically designed to support a wide range of applications, the FACSCalibur features a high level of automation (instrument control managed via software, digital fluidic control) to ensure the extreme reproducibility of the results obtained.

Spectral cytometer, equipped with 3 laser sources (488nm - blue, 633nm - red, 405nm – violet) that allows the simultaneous analysis of up to 30 parameters, 25 of which are fluorescence.

Compared to conventional flow cytometry, spectral flow cytometry facilitates multicolor analysis. It is based on many of the fundamental aspects of conventional flow cytometry but offers unique optical collection and analytical capabilities.

In spectral flow cytometry, the emission spectrum of each fluorescent molecule is captured by a set of detectors within a defined wavelength range. The Cytek® Northern Lights™ cytometer resolves different fluorochromes by exploiting the differences in their spectral emission signatures from all lasers, even in the case of co-expression of markers. Its optical design and unmixing algorithm provide significant flexibility, allowing easy use of a wide range of fluorochrome combinations, even when fluorochromes have highly overlapping peak emissions, without sacrificing resolution.

Additionally, in flow cytometry, the resolution of the fluorescence signal is inversely proportional to the autofluorescence content of the sample. Extracting autofluorescence becomes crucial for resolving weakly expressed markers in immunophenotyping analyses, minimizing inconsistencies arising from variations in intrinsic cellular autofluorescence. The Cytek® Northern Lights™ cytometer is capable of extracting sample autofluorescence, significantly facilitating the resolution of populations of interest.

TECHNICAL SPECIFICATIONS

• High-throughput spectral analyzer

• 3 lasers with wavelengths of violet (405nm), blue (488nm), and red (640nm)

• Digital acquisition up to 35,000 events/sec

• Multiparametric analysis up to 30 parameters

• Automated Sample Loader compatible with 96-well plates and 40-tube racks

High-performance cytometer and cell sorter, equipped with 4 laser sources (488nm - blue, 633nm - red, 405nm - violet, 375nm - near UV); it allows the simultaneous analysis of up to 13 parameters, 11 of which are fluorescence.

The number of lasers installed, combined with the revolutionary optical bench, allows for a significant expansion in the number of fluorescence signals the instrument can simultaneously analyze and, consequently, in the selection of usable fluorochromes.

The superior performance of the excitation system, fluorescence collection, electronics, and the FACSDiva 9 software, complemented by the ease of use of the instrument and the rapidity of setup due to the introduction of fixed alignment technology, which also ensures extremely high reproducibility, makes the FACSAria IIIu the cell sorter of choice in modern biomedical research laboratories.

TECHNICAL SPECIFICATIONS

• High-throughput cell sorter with a high-sensitivity square cuvette

• 4 lasers with wavelengths nearUV (375nm), violet (405nm), blue (488nm), and red (633nm)

• Digital acquisition up to 70,000 events/sec

• Sorting speed up to 20,000 events/sec

• Multiparametric analysis up to 12 parameters

• Nozzles: 70μm, 85μm, 100μm, and 130μm

• Sorting capability to 4-way in 1.5ml Eppendorf tubes or 4ml tubes

• Single-cell sorting in 6-, 24-, 96-, and 384-well plates or microscope slides