Next Generation Sequencing

Next Generation Sequencing (NGS) is a technology that enables comprehensive and detailed genome analysis. This methodology makes it possible to identify genomic alterations, analyze gene expression profiles, and study the microbiome present in a sample (for example can be used for large-scale identification of microorganisms in water and food).

The services offered by the facility can be applied across all these following fields

• Genomic analysis: NGS allows for the sequencing of entire genomes, exomes (the coding regions of DNA), or specific gene panels. This technology enables the detection of point mutations, small insertions and deletions, chromosomal rearrangements, and copy number variations (CNVs). In-depth genome analysis provides essential information for genetic research and biomedical applications.

• Transcriptomics: one of the primary applications of NGS is gene expression analysis, allowing for the simultaneous quantification of thousands of genes. Compared to microarrays, NGS offers greater accuracy and depth, enabling the identification of novel genes and transcript isoforms and improving our understanding of transcriptome complexity.

• Microbiome profiling: NGS sequencing enables the characterization of microbial communities within a given environment, identifying imbalances associated with pathological conditions. At the facility, microbiome analysis is performed by sequencing the entire genome of the organisms present in the sample, allowing for the simultaneous identification of prokaryotic, eukaryotic, archaeal, and viral organisms.

The facility is equipped to analyze both human and non-human samples, providing expert data interpretation thanks to a team with extensive experience and numerous scientific publications. Additionally, a free consultation is offered before the experiment, ensuring a scientifically robust and efficient investigation with guidance on study design to optimize results while minimizing costs.