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Next Generation Sequencing

COMPETENCES

EQUIPMENTS

PRICES

PUBLICATION

CONTACTS

Equipments

The Genomics, Transcriptomics, and Microbiome Profiling Service employs cutting-edge technologies for the analysis of DNA, RNA, and the microbiome, supporting research projects in the biomedical, environmental, and biotechnological fields.

The instrumentation available in the Service's laboratories enables the development of a complete analytical workflow, including nucleic acid extraction, quality control, quantification, automated NGS library preparation, and sequencing using next-generation sequencers.

The combination of these advanced instruments and the specialized expertise within the Service ensures highly accurate results for the analysis of various sample types.

NextSeq 500

NGS sequencer that utilizes Sequencing by Synthesis (SBS) technology. Its fast run times, high throughput capacity, and flexibility make it ideal for a wide range of genomic applications.


TECHNICAL SPECIFICATIONS 

  • Sequencing technology: Sequencing by Synthesis (SBS)

  • Sequencing modes: Single-End / Paired-End

  • Generates up to 800 million reads per run (in paired-end mode)

  • Read length: up to 150 bp

  • Sequencing capacity: up to 120 Gb of data per run


Ion GeneStudio S5

NGS sequencer that utilizes semiconductor sequencing technology. It supports a variety of applications with different workflows, offering low operating costs and fast turnaround times, making it suitable for both research and clinical settings.


TECHNICAL SPECIFICATIONS

  • Sequencing technology: Semiconductor sequencing

  • Sequencing mode: Single-End

  • Generates up to 80 million reads per run

  • Read length: up to 400 bp

  • Sequencing capacity: up to 15 Gb of data per run


Maxwell RSC

Maxwell RSC is an automated nucleic acid extraction system suitable for a wide range of applications and biological samples. It ensures fast, reliable, and high-quality nucleic acid extraction.


TECHNICAL SPECIFICATIONS 

  • Extraction technology: magnetic particle-based extraction

  • Capable of extracting nucleic acids from various sample types, including blood, tissues, saliva, oral swabs, cell cultures, and more

  • Extraction time: 30–60 minutes, depending on sample type and protocol used

  • Sample capacity per run: 1 to 16 samples simultaneously


Ion Chef System

The Ion Chef system is an advanced, fully automated solution for NGS library preparation, designed for research laboratories handling high sample volumes or requiring a high-throughput workflow. It is a versatile and scalable system, compatible with a wide range of NGS applications, from whole-genome sequencing to targeted library preparation.


TECHNICAL SPECIFICATIONS 

  • Sample capacity per run: supports up to 12 samples per run, depending on the model and protocol used

  • Sample types: compatible with a wide range of samples, including genomic DNA, RNA, and DNA/RNA from liquid biopsies


NanoDrop ND-1000

A microvolume UV-Vis spectrophotometer designed for measuring DNA, RNA, and protein samples with minimal sample volumes.


TECHNICAL SPECIFICATIONS

  • Sample volume: 1 µL

  • Spectral range: 220–750 nm

  • Wavelength accuracy: ±1 nm

  • Absorbance accuracy: ±3% (at 0.74 A at 350 nm)

  • Absorbance repeatability: ±0.002 A (at 0.7 A)

  • Detection limit (dsDNA): 2 ng/µL

  • Maximum measurable concentration (dsDNA): 3700 ng/µL


2100 Bioanalyzer / Tapestation 4150

Automated electrophoresis systems used for quality control of DNA and RNA samples. They are designed to analyze size, quantity, and integrity of a sample, making them ideal for workflows such as Next-Generation Sequencing (NGS) and biobanking, providing highly precise analytical assessment.


TECHNICAL SPECIFICATIONS 

Sample types analyzed: total RNA, small RNA, DNA

Sensitivity – 2100 Bioanalyzer:

  • RNA: 50 pg/µL in water or 200 pg/µL in TE

  • Small RNA: 50 pg of purified miRNA sample or 10 ng of total RNA

  • DNA: up to 5 pg/µL for fragment analysis; 100 pg/µL for complex DNA samples, such as NGS libraries


Sensitivity – 4150 TapeStation:

  • DNA: detection limit of 5 pg/µL for DNA fragments

  • RNA: detection limit of 100 pg/µL for total RNA


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