Pkiwi Lab 

Intro:

In this project, we were asked to explore the term GMO through a procedure called bacterial transformation. We performed two bacterial transformation reactions on -DNA and +DNA on LB and LB/AMP plates. We then recorded the number of bacterial colonies that grew and observed what happened to the plates. Below, is more information on the lab and the results. 

Content:

GMO - genetically modified organism 

Bacterial transformation - putting new DNA into bacteria cells to give them a new trait 

Plasmid - small circular piece of DNA used to transform cells 

Genetic engineering - the process of changing an organism's genes using biotechnology to make something practical or useful 

Fluoresce - means "to glow"

Reflection:

This lab has taught me about my skills that I am exceeding at. First of all, I noticed that I was doing a good job at collaborating with my teammates. For example, I always made sure that the workload was split up and that my partners were on the same page as me. I also tried to get their perspective and opinion on the lab write up. This helped me to understand what I needed to change. I also learned that I have satisfactory communication skills. For example, I was one of the main planners in my group and made sure that everyone knew their roles and positions in the lab. I helped to communicate different ideas to my group. 

This lab has also taught me about some skills I need to work on. One skill I need to work on is my creativity. There were times in the project where my group was stuck and I just sat in confusion. Instead of doing this, I could share new ideas and think outside of the box for answers to problems. I also need to work on my critical thinking skills.  In the last week, my group was confused about the term fracking and how to do it. Instead of asking Ms. Mathews for help right away, I could have used other resources to get the information needed. Throughout the next project, I am going to prioritize these skills and do whatever it takes to improve them.