Protocols

Cloning and mutagenesis

References

Akada R et al. 2002. Sets of integrating plasmids and gene disruption cassettes containing improved counter-selection markers designed for repeated use in budding yeast. Yeast 19, 393-402.

Alani E et al.1987. A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains. Genetics. 116, 541-545.

Cao Y et al. 2008. In vivo reconstruction of autophagy in Saccharomyces cerevisiae. J Cell Biol. 182(4), 703-713. Multigene plasmids construction.

(*)Gueldener U et al. 2002. A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast. Nucleic Acids Research. 30(6), e23. Efficient method for gene knockout (*).

Lindbladh C et a. 1994. Matabolic studies on Saccharomyces cerevisiae containing fused citrate synthase/malate dehydrogenase. Biochemistry, 33, 11684-11691.

(*)Longtine MS et al. 1998. Yeast functional analysis reports. Yeast 14, 953-961. Efficient method for gene knockout (*).

Oldenburg KR et al. 1997. Recombination-mediated PCR-directed plasmid construction in vivo in yeast. Nucleic Acids Research, 25(2), 451-452.

Sikorski RS and Hieter P. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics. 122, 19-27.

(*)Zheng L et al. 2004. An efficient one-step site-directed and site-saturation mutagenesis protocol. Nucleic Acids Research. 32(14), e115. Efficient methods for quick mutagenesis change (*).