2022
Nakamura, M., Fujiwara, K., Doi, N.: Cytoplasmic delivery of siRNA using human-derived membrane penetration-enhancing peptide. J. Nanobiotechnol. 20, 458 (2022)
Takada, S., Yoshinaga, N., Doi, N., Fujiwara, K.: Controlling the Periodicity of a Reaction-Diffusion Wave in Artificial Cells by a Two-Way Energy Supplier. ACS Nano 16, 16853-16861 (2022)
Takada, S., Yoshinaga, N., Doi, N., Fujiwara, K.: Mode selection mechanism in traveling and standing waves revealed by Min wave reconstituted in artificial cells. Sci. Adv. 8, eabm8460 (2022)
Ide, M., Tabata, N., Yonemura, Y., Shirasaki, T., Murai, K., Wang, Y., Ishida, A., Okada, H., Honda, M., Kaneko, S., Doi, N., Ito, S., Yanagawa, H.: Guanine nucleotide exchange factor DOCK11-binding peptide fused with a single chain antibody inhibits Hepatitis B Virus infection and replication. J. Biol. Chem. 298, 102097 (2022)
Suzuki, M., Iwaki, K., Kikuchi, M., Fujiwara, K., Doi, N.: Characterization of the membrane penetration-enhancing peptide S19 derived from human syncytin-1 for the intracellular delivery of TAT-fused proteins. Biochem. Biophys. Res. Commun. 586, 63-67 (2022)
2021
Matsui, Y., Akui, T., Doi, N., Fujiwara, K.: Activation of a diluted E. coli cell-free transcription-translation system within liposomes by hypertonic concentration. STAR Protoc. 2, 101003 (2021)
Deyama, T., Matsui, Y., Chadani, Y., Sekine, Y., Doi, N., Fujiwara, K.: Transcription-translation of the Escherichia coli genome within artificial cells. Chem. Commun. 57, 10367-10370 (2021)
Akui, T., Fujiwara, K., Sato, G., Takinoue, M., Nomura, S.M., Doi, N. System concentration shift as a regulator of transcription-translation system within liposomes. iScience 24, 102859 (2021)
Uwaguchi, Y., Fujiwara, K., Yoshinaga, N., Doi, N.: Switching ON of transcription-translation system using GUV fusion by co-supplementation of calcium with long-chain polyethylene glycol. ChemBioChem 22, 2319-2324 (2021)
Takahashi, K., Sato, G., Doi, N., Fujiwara, K.: A relationship between NTP and cell extract concentration for cell-free protein expression. Life 11, 237 (2021)
2020
Kohyama, S., Fujiwara, K., Yoshinaga, N., Doi, N.: Self-organization assay for Min proteins of Escherichia coli in micro-droplets covered with lipids. Bio Protoc. 10, e3561 (2020)
Kohyama, S., Fujiwara, K., Yoshinaga, N., Doi, N.: Conformational equilibrium of MinE regulates the allowable concentration ranges of a protein wave for cell division. Nanoscale 12, 11960-11970 (2020)
Goto, H., Kanai, Y., Yotsui, A., Shimokihara, S., Shitara, S., Oyobiki, R., Fujiwara, K., Watanabe, T., Einaga, Y., Matsumoto, Y., Miki, N., Doi, N. Microfluidic screening system based on boron-doped diamond electrodes and dielectrophoretic sorting for directed evolution of NAD(P)-dependent oxidoreductases. Lab Chip 20, 852-861 (2020)
2019
Yoshida, A., Kohyama, S., Fujiwara, K., Nishikawa, S., Doi, N. Regulation of spatiotemporal patterning in artificial cells by a defined protein expression system. Chem. Sci. 10, 11064-11072 (2019)
Kohyama, S., Yoshinaga, N., Yanagisawa, M., Fujiwara, K., Doi, N. Cell-sized confinement controls generation and stability of a protein wave for spatiotemporal regulation in cells. eLife 8, e44591 (2019)
濱野理, 土居信英. mRNAディスプレイによる翻訳アレスト配列の大規模探索. 生物工学 97, 10-12 (2019)
2018
Aye, S.L., Fujiwara, K., Doi, N. A dual system using compartmentalized partnered replication for selection of arsenic-responsive transcriptional regulator. J. Biochem. 164, 341-348 (2018)
Aye, S.L., Fujiwara, K., Ueki, A., Doi, N. Engineering of DNA polymerase I from Thermus thermophilus using compartmentalized self-replication. Biochem. Biophys. Res. Commun. 499, 170-176 (2018)
Fujiwara, K., Adachi, T., Doi, N. Artificial cell fermentation as a platform for highly efficient cascade conversion. ACS Synth. Biol. 7, 363-370 (2018)
土居信英. 新規の膜透過促進ペプチドを利用した抗体医薬の細胞選択的 DDS に向けて. 「ドラッグデリバリーシステム―バイオ医薬品創成に向けた組織、細胞内、核内送達技術の開発―」シーエムシー出版, pp. 131-137 (2018)
2017
Fujiwara, K., Sawamura, T., Niwa, T., Deyama, T., Nomura, S.M., Taguchi, H., Doi, N. In vitro transcription-translation using bacterial genome as a template to reconstitute intracellular profile. Nucleic Acids Res. 45, 11449-11458 (2017)
Sudo, K., Niikura, K., Iwaki, K., Kohyama, S., Fujiwara, K., Doi, N. Human-derived fusogenic peptides for the intracellular delivery of proteins. J. Control. Release 255, 1-11 (2017)
Kurokawa, C., Fujiwara, K., Morita, M., Kawamata, I., Kawagishi, Y., Sakai, A., Murayama, Y., Nomura, S.M., Murata, S., Takinoue, M., Yanagisawa, M. DNA cytoskeleton for stabilizing artificial cells. Proc. Natl Acad. Sci. USA 114, 7228-7233 (2017)
土居信英. タンパク質の細胞質送達を促進するヒト由来膜融合ペプチド. 「医療・診断をささえるペプチド科学―再生医療・DDS・診断への応用―」シーエムシー出版, pp. 232-238 (2017)
土居信英. ヒト由来膜融合ペプチドによるバイオ医薬の DDS. 細胞 49, 602-605 (2017)
2016
Nakayama, M., Komiya, S., Fujiwara, K., Horisawa, K., Doi, N. In vitro selection of bispecific diabody fragments using covalent bicistronic DNA display. Biochem. Biophys. Res. Commun. 478, 606-611 (2016)
Fujiwara, K., Doi, N. Biochemical preparation of cell extract for cell-free protein synthesis without physical disruption. PLoS ONE, 11, e0154614 (2016)
Nagumo, Y., Fujiwara, K., Horisawa, K., Yanagawa, H., Doi, N. PURE mRNA display for in vitro selection of single-chain antibodies. J. Biochem. 159, 519-526 (2016)
Niikura, K., Horisawa, K., Doi, N. Endosomal escape efficiency of fusogenic B18 and B55 peptides fused with anti-EGFR single chain Fv as estimated by nuclear translocation. J. Biochem. 159, 123-132 (2016)
2015
Niikura, K., Horisawa, K., Doi, N. A fusogenic peptide from a sea urchin fertilization protein promotes intracellular delivery of biomacromolecules by facilitating endosomal escape. J. Control. Release 212, 85-93 (2015)
Morita, M., Onoe, H., Yanagisawa, M., Ito, H., Ichikawa, M., Fujiwara, K., Saito, H., Takinoue, M. Droplet-Shooting and Size-Filtration (DSSF) Method for Synthesis of Cell-Sized Liposomes with Controlled Lipid Compositions. ChemBioChem 16, 2029-2035 (2015)
Yamashita, H., Morita, M., Sugiura, H., Fujiwara, K., Onoe, H., Takinoue, M. Generation of monodisperse cell-sized microdroplets using a centrifuge-based axisymmetric co-flowing microfluidic device. J. Biosci. Bioeng. 119, 492-495 (2015)
2014 （IN 藤原慶；OUT 堀澤健一）
Oyobiki, R., Kato, T., Katayama, M., Sugitani, A., Watanabe, T., Einaga, Y., Matsumoto, Y., Horisawa, K., Doi, N. Toward high-throughput screening of NAD(P)-dependent oxidoreductases using boron-doped diamond microelectrodes and microfluidic devices. Anal. Chem. 86, 9570-9575 (2014)
Nagata, T., Shirakawa, K., Kobayashi, N., Shiheido, H., Tabata, N., Sakuma-Yonemura, Y., Horisawa, K., Katahira, M., Doi, N., Yanagawa, H. Structural basis for inhibition of the MDM2:p53 interaction by an optimized MDM2-binding peptide selected with mRNA display. PLoS ONE 9, e109163 (2014)
Yanagisawa, M., Nigorikawa, S., Sakaue, T., Fujiwara, K., Tokita, M. Multiple patterns of polymer gels in microspheres due to the interplay among phase separation, wetting, and gelation. Proc. Natl Acad. Sci. USA 111, 15894-15899 (2014)
Fujiwara, K. (corresponding author), Yanagisawa, M., Nomura, S.M. Reconstitution of intracellular environments in vitro and in artificial cells. BIOPHYSICS 10, 43-48 (2014)
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2005-2013
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