Molecular Protocols

This page contains guides to basic genetic and molecular protocols in the Marder lab.

Pipette Tip Recycling Box

We have a pipette tip recycling box in the lab located by the fume hood in the main lab. Green Labs Recycling is run by Brandeis Grad Alums.

General Brain Collection

Pestle Cleaning Protocol

For cleaning the reusable blue pestles used for homogenizing samples as part of the RNA extraction protocol.

RNA extraction through qPCR or PCR

• Contains protocols for the following procedures: RNA extraction, cDNA Synthesis, DNA Purification, qPCR, Endpoint (regular) PCR, Gel Electrophoresis, and dsRNA production.

• Both the abbreviated and expanded version are below

RNA Extraction thru NanoDrop Worksheet

• Used to document RNA extractions and cDNA synthesis in lab notebooks.

RNA from Fibrous Tissues

Single Cell Collection for RNA-seq

Western Blotting

Misc. Protocols