Buffers

Phosphate buffer recipes can be found below and on note cards taped to the shelf near the scales/pH meter.

• At all times, we keep 1L of regular PBS and 1L of PBS-T (with Triton added).

• The stock solutions for the buffers are kept in the cold room and are replaced when we run out. The recipes for the stocks are in the same location.

PBS 1x

• Per 1 L H2O

  • • 440 mM NaCl X M/1000mM X 58.44 g/mol = 25.71 g NaCl

    • 11 mM KCl X M/1000mM X 74.55 g/mol = 0.82g KCl

    • 10 mM Na2HPO4 X M/1000mM X 141.96 g/mol = 1.4196 g Na2HPO4

    • 2 mM KH2PO4 X M/1000mM X 136.09 g/mol = 0.272 g KH2PO4

• Per 9 L H2O (make this!)

  • • 231.4 g NaCl

    • 7.38 g KCl

    • 12.78 g Na2HPO4

    • 2.448 g KH2PO4

**Adjust pH to 7.4 (add approximately 800 μL of 10M NaOH)

PBS-T 0.1%

• Follow the procedure above.

• At the end, add 1mL Triton X-100 per L H2O.

• For the standard 9L solution, add 9 mL Triton X-100. **Pipette the Triton very slowly to avoid loss/bubbling.**

  • • Monobasic: 0.1 M = (55.196 g NaH2PO4 *H2O)/(4L dH2O)

    • Dibasic: 0.1 M = (56.784 g Na2HPO4)/(4L dH2O)

For a more complete list, see the following recipes from the NJIT STG Lab website.