To avoid unevenly illuminated samples, it is important to carefully align Köhler Illumination.
Detailed instructions can be found here.
The alignment of the illumination can be checked easily after starting up the manualWSI software.
After bringing the sample into focus, simply reduce the size of the field iris diaphragm until the image on the camera is reduced.
The screenshot below shows a well aligned illumination. The bright spot is in the center of the camera image and the edges of the field iris diaphragm are "sharp". If the image looks significantly different, please follow these instructions to align Köhler illumination.