It is possible to use items on Minish Portals (tree stumps, pots, stones, dungeon portals). All items except for the Gust Jar and the Roc's Cape work.
How to:
Position yourself next to a portal*
Push against the portal while walking diagonally into it (For example, left side: hold up+right, from above: hold down+left or down+right)
Use and item and hold the button for the item
After a few frames, start only holding the direction towards the portal (For example: left side: start holding right, from above: start holding down)
Link will use the item once, jump onto the portal, and then use it a second time
*For some items only specific sides work, for example, to use the Ocarina on a portal, you have to perform Portal Items from above a portal. For most items, however, the side you perform Portal Items on doesn't matter.
Depending on the items you use, a few different things can happen.
For most of them, the item will be used once on the ground, and then a second time on the portal. Note that something in a bottle will not disappear until the second use, so you can technically turn one Fairy into two.
The Mole Mitts will put Link a bit more upwards on stones and dungeon portals due to the knockback. This can be used to jump onto a wall in Fortress of Winds.
Bonking on a Portal with the Pegasus Boots will push Link from the Portal, but some of the effects from being on a Portal don't disappear:
Standing still will put Link into the animation he gets when standing on a Portal
You cannot leave the area anymore except for very few cases
Enemies stop noticing you
The Ocarina bird cannot pick you up anymore
In order to use items, you need to press R+Item
You cannot shrink anymore
Using a bottle will allow Link to take damage on the portal. Dying will put Link into the same state as bonking with the Pegasus Boots.
Uses for Portan Items: Fortress of Winds Big Key Skip.
After getting the Ocarina Glitch and still being on the frozen screen, jumping onto a portal will (almost always) make Link jump at an angle, instead of jumping directly onto it. By doing Portal Items using the Ocarina or by just simply jumping onto the portal from above, Link will be far enough away from the Portal to not be able shrink by pressing R after cancelling the Ocarina Glitch. Using this, you can move around by using the Gust Jar on the Portal by pressing R+Gust Jar at the same time (works with every item). Since Link is still technically on the Portal, pressing any directions will make Link jump off of it. Usually, Link will not cover any distance by doing this, however, if there is a wall (or anything else solid) next to Link, he will jump over it. You can also increase the jump's range by a tiny bit by either taking damage or using the Roc's Cape once.
Originally thought to require Portal items, however it only requires the Ocarina Glitch. It allows you to move on Minish Portals, which in turn allows you to jump over any solid object. More information is available on its own page: Portal Jump Storage