Results and Conclusions

Results

Graphed Average Weekly Trials 

Anova Test on Both Sets of Trials  

Conclusion

Our ANOVA analysis showed a high p-value between our week 1 and 2 data sets for vglutGAL4 and vglutGAL4>TDP43. Given how our threshold is at 0.05, there is not enough evidence to suggest a significant difference in flies. However, this ANOVA is specifically tested at a time point of 120 seconds. Due to difficulties regarding ANOVA, it could be concluded that in earlier time points (10-110 seconds) there would be a significant difference in flies that climbed over 10-110 seconds. This would suggest the idea of muscles in the flies weakening over age as younger flies are seen to climb up the graduated cylinder (fly locomotor test) in the earlier timeframe. This idea is supported by our graphs of each graph (vlgutGAL4 week 1-2, and vglutGAL4>TDP43 week 1-2).
vlgutGLA4;+ flies week 2 flies were seen to have the worst performance in the locomotor test. This was not originally anticipated this result as we thought that TDP43 is active in ALS development which should have shown a worse locomotive trial performance. However, the vlgutGAL4;+ week 2 gene had the worst performance in the locomotive test. 

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