Results

Gathered Data

Recorded Data of Locomotor Trials

This graph reflects our locomotor behavior testing results based on our Method(s) section. As so, the given graph highlights various subcategories of behavior testing, including: strain name, data of test, birthdate, age, type of test in mL, # of flies, sex, and any moralities. Below the subcategories, is an account of flies that were seen to climb based on 10 second intervals for each locomotor test. 

In this trial, vglutGAL4 behavior was seen to be at an all time high around week 3 as the percentage of flies that climbed remained superior than the other weeks. 

In this trial, TDP43/vglutGAL4 behavior was seen to be the highest around week 2, with a steady 100% of flies climbing. 

Coding for ANOVA

Finally to make sense of our data and give it significance, we used ANOVA in order to calculate the p value and the F statistic in order to determine whether there was a significant difference among the week columns. This picture displays the code used in order to calculate standard deviation for each column in the top table, as well as displays the code used in order to calculate the p value and f statistic

After inputting the data gathered on vglutGAL4 flies, the code above produced the following values. The code calculated the standard deviation for each column related to vglutGAL4 flies. Additionally, the code produced the f statistic as well as the p value, alerting us that there was a significant difference among the columns.

The values to the left were produced after inputting the data on TDP43 flies. The code calculated the standard deviation for each week related to TDP43 flies. The code also produced the f statistic and the p value to signify that there was a significant difference among the columns. 

TIMP Behavior Trial

The graph to the right is the result of three separate trials ran on week one flies. All the data was averaged for week one to produce the following graph. In comparison to the other week one flies such as TDP43 and vglutGAL4, TIMP flies performed worse than TDP43, however performed similarly to vglutGAL4 flies but slightly better due to the significant increase in fly quantity. Further testing of week 2 and week 3 TIMP flies are needed to conclude which genotype performed the worse.