Digital and Confocal Microscopy

An automated station for digital imaging of cytometric parameters in living cells. Automatic object recognition allows the use of high-content/high-throughput protocols for key cellular events (apoptosis, necrosis, autophagy/mitophagy, cell cycles, morphological changes, protein expression, automated FISH analysis, and protein localization and translocation). 

High-resolution fluorescence microscopy system with multiple wavelengths. The system's high resolution enables 2D/3D analysis of intracellular structures and organization (e.g., mitochondria, endoplasmic reticulum, and cytoskeleton). Additionally, the multiple wavelengths and the system's versatility allow real-time measurements of ratiometric probes such as Fura or Pericam. 

High-speed confocal fluorescence microscopy system for in vivo analysis of multiple cellular parameters. The system allows simultaneous measurement of cellular parameters such as calcium concentration (cytoplasmic/mitochondrial), protein translocation, reorganization of cellular structure, and vesicle generation, loss, and fusion. Three excitation lines are equipped, based on solid-state lasers (488 nm, 561 nm, 635 nm), along with two different sets of emission filters (488/561, 488/643). This system enables the simultaneous visualization of two different fluorophores 

The Seahorse XF96 Extracellular Flux Analyzer simultaneously measures mitochondrial respiration and glycolysis in cells in real-time. This instrument provides the most physiologically relevant in vitro measurement for your cellular metabolism research in primary cells and tumor cell lines, both adherent and in suspension, as well as isolated islets and mitochondria. It utilizes plates calibrated with fluorescent compounds to simultaneously measure oxygen levels and pH in the medium. By measuring key parameters of cellular metabolism, it provides functional data that enhance the understanding of cancer, aging, and metabolic, cardiovascular, and neurodegenerative diseases. 

Manual system for digital and confocal imaging of mitochondrial parameters in live animals (laboratory mice). The use of high-power LEDs and confocal acquisition capabilities allows the detection and analysis of probes located in tissues or tumor masses, several microns below the objective lens. The multi-wavelength acquisition allows live measurements of ratiometric probes such as FURA red and JC-1. In vivo experiments can be conducted on mitochondrial membrane potential, intracellular calcium flux (mitochondrial and cytosolic), and mitochondrial morphology.

TECHNICAL SPECIFICATIONS

Filterset and camera:

• CrEST with spectral profile DAPI/FITC/ORANGE/RFP and Photometrics EVOLVE camera
  
  

Magnifications:

• LUMPLFLN 40X W, Numerical aperture: 0.8, Working distance (mm): 3.3

• UPLFLN 10X, Numerical aperture: 0.3, Working distance (mm): 10
  
  

Environmental control:

• Thermostat for small rodents with digital temperature control
  
  

Microinjector:

• Eppendorf Transjector for microinjection of molecules in situ (in the tumor mass or a specific anatomical area of the anesthetized animal). It features an integrated pressure/vacuum system that allows for the collection, retention, and release of any substance (drugs, chemicals, probes, and oocytes).