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Do not allow the column to run dry!
Do not allow the column to run dry!
To avoid poor separation of the components, make sure the level of solvent does not go below the level of the silica. While some solvent still remains on top of the column bed, switch to the next test tube, and carefully add the next portion.
To avoid poor separation of the components, make sure the level of solvent does not go below the level of the silica. While some solvent still remains on top of the column bed, switch to the next test tube, and carefully add the next portion.
ASP_F1F2_clock_AC_A_3.mp4Slowly swirl the pipet around the inside walls of the column while gently applying pressure to the bulb
Collect the eluent in the labeled test tubes, ~15 mL of solvent per fraction
Great! Fractions F1 and F2 have been collected...
Great! Fractions F1 and F2 have been collected...
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