Embedded Files
SAFETY: Hexanes, ethyl acetate, and acetone are irritants and flammable... TLC chambers should be kept in the fume hoods and covered at all times.
Thin-layer chromatography (TLC) is used to assess the purity of each fraction
Thin-layer chromatography (TLC) is used to assess the purity of each fraction
The experimental videos of the microcolumn running showed ideal scenarios in terms of how the components are separated. However, we don't know how successful the separation was until we analyze each fraction via TLC. These results will help us make the next step (isolate), and provide insight on how to improve next time!
ASP, ACE, and CAF are colorless under visible light (~400-700nm), but since the molecules have conjugated pi systems, these compounds will absorb UV light (~200-400nm). The spots are circled on the TLC plate to help calculate retention factor (Rf) values.
same TLC plate with and without UV lamp
Discuss (IN-LAB #6):
Discuss (IN-LAB #6):
What is the mobile phase used for this TLC analysis? (HINT – same solvent mixture used in Revell, K. D. J. Chem. Ed. 2011, 88, 1413).
What role does the acid play as part of the TLC mobile phase?
Page updated
Report abuse