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SAFETY: Hexanes, ethyl acetate, and acetone are irritants and flammable... TLC chambers should be kept in the fume hoods and covered at all times.
TLC_F1-3_spot.mp41. Spot TLC plate
1. Spot TLC plate
Mark origin, then quickly spot the plate to keep the spot small (~3 spots per plate)
Rinse capillary tube with acetone to prevent cross-contamination
TLC_F1-3_run.mp42. Place TLC plate in chamber
2. Place TLC plate in chamber
Use tweezers to carefully place the TLC plate into the developing chamber
Allow to run until the solvent is ~1 cm from the top
Quickly mark solvent front on the plate
*repeat for fractions F4, F5, and F6*
Discuss:
Discuss:
Why should we avoid marking the TLC plate with pen before running?
What will happen if the spots are submerged in the mobile phase?
RESULTS: thin-layer chromatography (TLC) plates of column fractions (IN-LAB #1)
RESULTS: thin-layer chromatography (TLC) plates of column fractions (IN-LAB #1)
TLC plate of fractions F1, F2, and F3 with UV lamp OFF (left) vs. UV lamp ON (right).
TLC plate of fractions F4, F5, and F6 with UV lamp OFF (left) vs. UV lamp ON (right).
Discuss (Part of IN-LAB #2):
Discuss (Part of IN-LAB #2):
Which fractions are pure vs. impure?
With that in consideration:
Which fractions should be combined and concentrated?
Which fractions should be concentrated individually?
Which fractions should be discarded?
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