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SAFETY: Silica is an irritant... prevent spills and carefully transfer to RBF. Hexanes, ethyl acetate, and acetone are irritants and flammable... prepare solvents/solvent mixtures and load/run the column in the fume hood.
STATIONARY PHASE = Silicon dioxide, SiO2 (silica)
Diagram of the microcolumn (above)
prep_column.mp41. PREPARE the column
1. PREPARE the column
Label six large test tubes (fractions)
Clamp a disposable polypropylene column to a ring stand (check that there's a filter toward the bottom)
Add ~3 g of silica to the column using a powder funnel
pack_column.mp42. PACK & LOAD the column
2. PACK & LOAD the column
Add Excedrin-silica mixture using a powder funnel
Add a level, ~2 mm layer of sand
Subtract 1 mg from each component (ASP, ACE, and CAF).
Discuss:
Discuss:
What do you think could go wrong in the packing and loading of the column?
Explain the reason for product loss at this step (part of IN-LAB #4)
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