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SAFETY:Hexanes, ethyl acetate, and acetone are irritants and flammable... prepare solvents/solvent mixtures and load/run the column in the fume hood.
Discuss:
Discuss:
What is the polarity of the stationary phase (silica)?
Three mobile phases are used in this experiment: (least to most polar):
Three mobile phases are used in this experiment: (least to most polar):
30 mL of 1:1 Hex/EtOAc
30 mL of 1:2 Hex/EtOAc
30 mL of Acetone
Which mobile phase should be added to the column first?
OR
OR
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