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Carefully add the first portion of the mobile phase!
Carefully add the first portion of the mobile phase!
To avoid disturbing the stationary phase, it's a good practice to slowly pipet solvent/solvent mixture (mobile phase) onto the column instead of pouring it directly onto the sand. Once the solvent level is high enough, the amount remaining can be added more quickly.
To avoid disturbing the stationary phase, it's a good practice to slowly pipet solvent/solvent mixture (mobile phase) onto the column instead of pouring it directly onto the sand. Once the solvent level is high enough, the amount remaining can be added more quickly.
ASP_F1F2_clock_AC_A_1:2.mp4Slowly swirl the pipet around the inside walls of the column while gently applying pressure to the bulb
Collect the eluent in the labeled test tubes, ~15 mL of solvent per fraction
Discuss:
Discuss:
What would happen if you poured in the first solvent portion quickly?
Why should we avoid disturbing the stationary phase (column bed)?
What's happening as the first fraction is collected?
Once a majority of the first 15 mL fraction (F1) is obtained, how should we proceed...
Once a majority of the first 15 mL fraction (F1) is obtained, how should we proceed...
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