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SAFETY: Hexanes, ethyl acetate, and acetone are irritants and flammable... TLC chambers should be kept in the fume hoods and covered at all times. Aspirin and acetaminophen are irritants, and caffeine is a stimulant... prevent spills and carefully prepare the solutions of the standards.
prep_standards.mp41. Prepare solutions
1. Prepare solutions
Place a small amount of each standard (ASP, ACE, and CAF) in separate test tubes
Dilute with 1 mL of acetone
TLC_standards_spot.mp42. Spot TLC plate
2. Spot TLC plate
Mark origin, then quickly spot the plate to keep the spot small (~3 spots per plate)
Rinse capillary tube with acetone to prevent cross-contamination
TLC_standards_run.mp43. Place TLC plate in the chamber
3. Place TLC plate in the chamber
Use tweezers to carefully place the TLC plate into the developing chamber
Allow to run until the solvent is ~1 cm from the top
Quickly mark solvent front on the plate
Discuss:
Discuss:
What happens if the spot is too dilute? Too concentrated?
Why should we mark the solvent front as soon as the plate is removed from the developing chamber?
RESULTS: thin-layer chromatography (TLC) plate of standards (IN-LAB #1)
RESULTS: thin-layer chromatography (TLC) plate of standards (IN-LAB #1)
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