1:2 Hex / EtOAc

ACE_F3 & F4.mp4

Slowly swirl the pipet around the inside walls of the column while gently applying pressure to the bulb
Collect the eluent in the labeled test tubes, ~15 mL of solvent per fraction

Discuss:

Which technique will we use later to verify what's in each fraction?

Awesome! Now fractions F3 and F4 have been collected.

NEXT - add the most polar solvent!

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