Two Candidates re SARS-2 Origin.
Zhiyan-Le, 2021-04-29.
https://zhiyanleback.blogspot.com/p/two-candidates-re-sars-2-origin.html
https://sites.google.com/site/zhiyanleback/2021-1/z20210429-two-origins
Key Words: Science and research communications, SARS-2, Covid-19.
There are two possible candidates regarding the SARS-2 origin. One is bat RaTG13, as the WHO-China joint report said. The other is WIV1 series (Wuhan Institute of Virology, No.1 virus. Totally, it has 8 samples, plus WIV16). The following are key info/data suggesting that RaTG13 is too good to be true as the SARS-2 origin and that WIV1 is the real origin.
Samples for this essay:
Note: If RaTG13 were really a natural bat sample, its nCov should behave as the same, or at least very close to, how the other two natural bat samples do.
And here is the alignment on single-gene level:
Taking WH-01(NC_045512) as the basic sample, RaTG13 has highest identities, and much higher than that of two natural bat sample (2012 and 2014). Question: in the same location (PRC, Yunnan Province) and in just one year, how come in nature that the nCov in RaTG13 could be mutated so close to a patient sample which will be coming over 6.5 years later? However, a lab can do it in 10 days.
And here are similarities on Codon-level:
Again, the similarity score of RaTG13 goes much, much higher than that of natural bat samples and, for the codon ATG, RaTG13 has it exactly as the same as what the Wuhan patient (NC_045512) has. Is this possible in natural mutations? Obviously not.
The RaTG13 sample has two versions: one is issued at 2020-03-24 and the new version is issued at 2020-11-24. And here is their aligned result by NIH Blast:
That is blasted on single-gene level. It shows a 99% identity with a few allele changes and a 15-bp gap (ATTAAAGGTTTATAC). To summarize:
In the above, BC refers to Basic Codon, of which each has its own 6 hidden codons. Hidden codon value refers to energy level corresponding to each codon.
It shows that RaTG13 version 2 gained 207 units on gene-energy-level. Question is: How could the change and its energy gain naturally happen as the WIV leaders said that they have genomic sequence without live-sample regarding the bat RaTG13 nCov?
Regarding the 15-bp gap (ATTAAAGGTTTATAC), here is a comparison:
The genomic data comes from each sample (Poland+, NIH Blast-Search result: hundreds 100% identical samples re patients around the world. Taking the first one, Poland.).
Question: where does the 15-bp gap come from? How does it go exactly the same 15-bp gap come to both RaTG13 (with no live-sample) and patients around the world in almost the same time around 2020 autumn?
There is only one convincing answer to the above questions, that is, RaTG13 nCov is a lab product made for similarities or identities as close as possible to that of patient samples so as to cover up the real SARS-2 origin, such as WIV1.
Regarding the changes for RaTG13 gene data, here is the relevant info by USRTK:
RATG13: ALTERED DATASETS RAISE MORE QUESTIONS ABOUT RELIABILITY OF KEY STUDIES ON CORONAVIRUS ORIGINS.
Posted on December 29, 2020.
NO PEER REVIEW FOR ADDENDUM TO PROMINENT CORONAVIRUS ORIGINS STUDY?
Posted on December 18, 2020
URL: https://usrtk.org/tag/ratg13/
It says that, when updating RaTG13 genomic sequence data, the provider, PRC-WIV, did not give a clear reason, nor did they go through peer-review and other normal processes.
Speaking of WIV1, its identities (87% to basic sample) should be good enough as the SARS-2 origin. The provider PRC-WIV said that it is a bat-nCov sample. However, in international meetings, it was reviewed and approved as a lab-product. For example:
In the above re BP genes frequency and distribution on Codon level, WIV1 has the smallest distance from patient sample (NC_045512), while RaTG13 has three times bigger distance than that of WIV1 and obviously different from that of natural samples. See below:
In more detailed terms, WIV1 and RaTG13 behave the same way:
In the above, 1st-2nd-3rd refer to three base genes. In terms of Codons frequency and distribution, which in fact the virus and ACE2 run and interact with each other, WIV1 has the highest score, 0.99468, to that of patient sample (NC_45512), while RaTG13 has clearly lower ration and obviously different from natural bat samples.
In sum, between the two candidates of SARS-2 origin, WIV1 is the real one; RaTG13 is not the SARS-2 origin, nor is it a natural sample.
In March 2016, PNAS (publication by US National Academy of Science) published an article with editor’s notes about the WIV1. Please see:
This Issue, by PNAS March 15, 2016 113 (11) 2793-2795;
it warned that WIV1 is a lab nCov product, which can directly jump to humans and cause huge pandemic with global economic loses, can even change existing living styles. Now the warning is a big reality that the US and the whole world are facing.
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