透明化試薬について（シート顕微鏡）

蛍光生体イメージング室にて使用している主な透明化試薬は下記3つになります。

・CUBIC

Susaki EA¹, Tainaka K², Perrin D³, Kishino F⁴, Tawara T⁵, Watanabe TM⁶, Yokoyama C⁷, Onoe H⁷, Eguchi M⁸, Yamaguchi S⁹, Abe T¹⁰, Kiyonari H¹⁰, Shimizu Y¹¹, Miyawaki A¹², Yokota H⁵, Ueda HR¹³.

Whole-BrainImaging with Single-Cell Resolution Using Chemical Cocktails and ComputationalAnalysis

24 April 2014, Pages 726-739

Published:April 17, 2014•DOI:https://doi.org/10.1016/j.cell.2014.03.042•

・第2世代CUBIC

Kubota SI¹, Takahashi K², Nishida J², Morishita Y², Ehata S², Tainaka K³, Miyazono K⁴, Ueda HR⁵.

Whole-Body Profiling of Cancer Metastasis with Single-Cell Resolution.

Cell Rep. 2017 Jul 5;20(1):236-250. doi: 10.1016/j.celrep.2017.06.010.

・Sca/eS

Hama H¹, Hioki H², Namiki K¹, Hoshida T³, Kurokawa H¹, Ishidate F¹, Kaneko T², Akagi T⁴, Saito T⁵, Saido T⁵, Miyawaki A^1,3.

ScaleS: an optical clearing palette for biological imaging.

Nat Neurosci. 2015 Oct;18(10):1518-29. doi: 10.1038/nn.4107. Epub 2015 Sep 14.