Brooksville and Hudson Railroad

At its greatest extent, the CSX Brooksville Subdivision extended as far north as Waldo. Track from Waldo to Archer was built in 1861 by the Florida Railroad (later the Florida Central and Peninsular Railroad) which extended as far as Cedar Key. In 1890, track was built from Archer south to Early Bird. The Seaboard Air Line bought the FC&P network in 1900 and in 1911, the line was extended south of Early Bird to Inverness. 

In 1925, the Brooksville and Inverness Railway, a Seaboard subsidiary, was built connecting Inverness with the former Tampa Northern Railroad in Brooksville. This would create an additional route from northern Florida to the Tampa Bay region. 

After track from Archer to Cedar Key was abandoned in 1932, the full line from Waldo to Sulphur Springs was designated as the Brooksville Subdivision. The Seaboard Air Line became the Seaboard Coast Line Railroad in 1967 after merging with their former rival, the Atlantic Coast Line Railroad. In the combined network, the full line was largely redundant as the Atlantic Coast Line Railroad had a nearly parallel route as well as its own main line to Tampa. The Seaboard Coast Line abandoned the Brooksville Subdivision between Waldo and Brooksville in the 1970s. The line now ends near the Broco Quarry. However, the line's SR mileposts numbers remain as they did when the full line was intact. 

In 1980, the Seaboard Coast Line's parent company merged with the Chessie System, creating the CSX Corporation. The CSX Corporation initially operated the Chessie and Seaboard Systems separately until 1986, when they were merged into CSX Transportation.