9-2 IR-LEGO
Place: Room B66, Myodaiji, NIBB
Date of installation: Mar. 2010
Feature: IR-LEGO (Infrared laser-evoked gene operator system) is the system to induce gene expression of the local area in living cells using IR laser irradiation.
(Ref: Kamei et al. Nat. Methods 6, 79-81. 2009, (Pubmed)Deguchi et al. Dev. Growth Differ. 51, 769-775. 2009 (Pubmed))
Specification:
・Microscope: IX81 (inverted)
・IR laser: 1440-1480nm, 1W (HPU 10X09-001, Furukawa)
・Objectives: x20 and x40 (for IR irradiation), x4, x10, x20 x40 for Observation
・Filters: DAPI, GFP-BP, GFP-LP, RFP
・Camera: DP72
・Software: cellSens