9-2 IR-LEGO

Place: Room B66, Myodaiji, NIBB 

Date of installation: Mar. 2010 

Feature: IR-LEGO (Infrared laser-evoked gene operator system) is a system that induces gene expression in a local area of living cells using IR laser irradiation.

(Ref： Kamei et al. Nat. Methods 6, 79-81. 2009, (Pubmed) Deguchi et al. Dev. Growth Differ. 51, 769-775. 2009 (Pubmed))

Specification: 

・Microscope: IX81 (inverted) 

・IR laser: 1440-1480nm, 1W (HPU 10X09-001, Furukawa) 

・Objectives: 4x, 10x,x20 (for IR irradiation) ,40x (for IR irradiation) 

・Filters: DAPI, GFP-BP, GFP-LP, RFP 

・Camera: DP72 OrcaFusion

・Software: cellSens Metamorph