Place: Room No. 3-529, Yamate 3rd bldg. 5th Floor

Date of installation: Feb. 2013

Features:

Confocal imaging, Two-photon imaging (wavelength tunable), Z stack, Time-lapse, Photo stimulation, Large image (Tiling), Spectral imaging (unmixing) and Photon counting

Specification:

・Microscope:DMI6000CS (inverted)

・Laser (nm): 405 / 448 / 488 / 514 / 552

・Two-photon laser: tunable 680-1300 nm (InSight DeepSee)

・Scanner: FOV scanner, Resonant scanner

・Internal detector: MA PMT 1ch, HyD 3ch

・Non-Descanned Detector (NDD) for two-photon imaging: HyD 2ch

・Filter for NDD: DAPI/FITC, CFP/YFP, FITC/TRITC, FITC/TXR, TRITC/A633, mCherry/Cy5 details (pdf link)

・Objective: x10, 20MI, x20W IR, x40W, (x40IR), x63W, (x63Oil)

・Filter for visual observation: GFP-LP, DsRed-LP details (pdf link)

・Stage Incubator1: CO2 chamber; RT~50℃, 5%CO2 (STXG-WSKMX-SET, TOKAI HIT)

・Stage Incubator2: Thermo Plate: 4-60℃ (TP-CHSQ-C, TOKAI HIT)

*HyD: High sensitivity Hybrid Detector