1-1 Confocal and 2-photon microscope

Leica TCS SP8 MP (Upright type)

Place: Room No. 3-529, Yamate 3rd bldg. 5th Floor

Date of installation: Feb. 2013

Features:

Confocal imaging, Two-photon imaging (wavelength-tunable), Z stack, Time-lapse, Photo stimulation, Spectral imaging (unmixing), and photon counting


Specifications:

・Microscope: STP6000 (upright)

・Laser (nm): 488 / 552

・Two-photon laser: Wave length tunable 680-1300 nm (InSight DeepSee)

・Scanner: FOV scanner, Resonant scanner

・Internal detector for confocal and two-photon: MA PMT 1ch

・Non Descanned Detector (NDD) for two-photon imaging: HyD* 2ch

・Filter for NDD: DAPI/FITC, CFP/YFP, FITC/TRITC, FITC/TXR, TRITC/A633, mCherry/Cy5 details (pdf link)

・Objective: x5, x25W (non-cover)

・Filter for visual observation: GFP-LP, DsRed-LP details (pdf link)

*HyD: High sensitivity Hybrid Detector