MicroBiology Lab : Bacteria and Protists

Bacteria 

Part A : Light Microscopy 

Observe the bacteria under a compound microscope. Draw a diagram of the bacteria seen.  Label the magnification .  Label the bacteria as spiral, spherical, or rod shaped.

Not if Gram staining is present.  Give the Scientific name and/or the Genus and species name if labeled on the slide.  Two slides must be viewed.

PART B : Digital Microscopy and Oil Immersion Lenses

The slides will be set on the oil immersion lens.  Do not move to any other magnification.  This is the maximum for a light microscope.  A special oil has be applied to the slide.  This oil can not touch the other lenses. 

Take one photo of the slide.  Take at least one measurement of a cellular structure.

Label the slide with your name and the name of the microorganism.  Save it as a Google Photo.   On a separate computer, label structures and finalize your image.

Print your image in color.

PART C : Questions

1. Why is there a limit to the magnification of a light microscope to 1000x?  Web Research

2. Why can't the nucleus of a bacterial cell be seen?

3. Web Research : What are the symptoms caused by Salmonella  bacteria when humans ingest them on food?  Find an electron micrograph  of this bacterium.  Be sure to label it with the source and magnification.  Print it.

4. Web Research:  What is the disease caused by the spirochetes called Treponema pallidium?   What are the symptoms?  What are the treatments?    Find an electronmicrograph , label it , document the magnification and print it.

5. Web Research : What is the diameter and length of a typical bacterium?  Write this in micrometers and write it in scientific notation in meters.

6. Web Research : Give some background on Clostridium botulinum.