1. Supplies must be defrosted. They are stored frozen at -4 C
Petri Dish Preparation ;
Cool the agar to 60 C
1. Source plates (5) 10 mL Agar
2. 10 unlabeled plates = controls
Agar + Growth Additive
3. 20 x One Stripe Plates
Agar + Growth Additive + Ampicillin
4. 10 x Two Stripe Plates
Agar + Growth Additive + Ampicillin + IPTG
Store at room temperature
A new sterile 10 mL pipete is used for each plate type