Major Components:
Dispenser:General Overview
The primary purpose of the dispenser is to lower and raise the sampling capsule into and out of the water. It is used to interface between the operator in a kayak and the sampling capsule.
Lowers and raises the sampler up to 3-6 meter depths (~10-20 ft.)
Actuates cutting mechanism
Allows operator to interface with the sampler
Able to be used easily in a kayak
Final Design
A modular design with high density polyethylene (HDPE) tubing was used to avoid entanglement of the steel cords and to prevent early cutting or bag-closing actuation. The user can easily lower the sampler by grasping on the HDPE tubing. A modular design was used to be able to sample at a variety of depths.
Figure 1: Dispenser
Capsule Housing:General Overview
The main purpose of the capsule housing is to provide a rigid structure for the sampler and protect the algae sample during retrieval.
Must be rigid and durable
Able to withstand any collision with rocks or anything else without major damage
Able to allow water to flow through the surface of the capsule
Able to remain stable when on the sediment
Water resistant
Must be compatible with the cutting mechanism
Final Design
A circular capsule housing was chosen as the final capsule design due to its simplicity as well as its ease of fabrication. It consists of stainless steel collars on the top and bottom of the capsule with stainless steel columns connecting the collars together, as seen in Figures 2 and 3. In addition, a brass mesh help provide sturdiness to the capsule. The circular capsule is compatible with the chosen wire cutting design, is sturdy, and allows water to flow through it.
Figure 2: Capsule Housing
The main purpose of the cutting mechanism is to cut algae on the top and bottom of the sampling capsule so that the algae can be collected.
Takes consistent algae sample sizes
Able to sample in various sediments
Is able to cut algae without needing a large force (<30 N)
Final Design
A steel wire cutting mechanism was chosen. The cutting mechanism consists of a brass block and steel music wire. One piece of wire runs inside along the top and bottom circular sheet metal pieces in an 'O' shape. There are two holes in each of the brass blocks. One end of the wire goes through one hole while the other end goes through the other hole. One end of the wire is secured by a nut and bolt, while the other end is pulled to close the wire shut, as seen in Figure 4. Algae is cut between the brass block and cutting wire.
Cutting Mechanism:General Overview
Figure 3: Components of Capsule Housing
Figure 4: Wire + Brass Block Cutting Mechanism
In order to ensure that both the top and the bottom of the sampler close at the same time, a pulley is placed in the middle of the cutting wire. To actuate the cutting mechanism, the pulley is pulled. The pulley is connected to a steel cord through two swaged loops. The steel cord runs within the tubing and is pulled by the user to actuate the cutting mechanism. These parts can be seen in Figure 5.
Figure 5: Components of Sampler
PlaceholdersGeneral Overview
The main purpose of the placeholders are to hold both the cutting wire and the mesh bag in an open configuration around the inside of the sampling capsule before they are actuated.
Durable and have low friction
Able to withstand cutting wire/bag replacement with negligible deformation
Water resistant
Easily replaceable
Final Design
Ultimately, the Delrin placeholder was selected due to the simplicity and the effectiveness of the design. The Delrin was a low fiction material that required a small amount of force to actuate the cutting wire. In addition, since the sampler needed a double wire design, the Delrin placeholders easily accommodated this.
If time is not an issue, the L-shaped Delrin pieces would be replaced with bulkier square-shaped blocks for sturdier placeholder design. The blocks would be threaded to the appropriate screw size that is on the metal sheet so they could be mounted to the sheet. The other end would be slit across in two spaces to hold the cutting wire and mesh bag components in place.
Figure 6: Delrin Placeholder CAD
Replaceable Bags
General Overview
The main purpose of the placeholders are to hold both the cutting wire and the mesh bag in an open configuration around the inside of the sampling capsule before they are actuated.
Finely netted to contain samples, but still drain out water
Same height as capsule
Dense, taut material
Easily attachable to Delrin placeholders
Final Design
Thin, tulle material was chosen since it was flexible enough to close and stiff enough to hold its shape. The mesh, tulle material was chosen for easy water drainage. In addition, tulle's semi-stringent properties makes for easy bag insertion and allows the bag to conform the cylindrical capsule shape and maintain this shape until closed shut. The material can be seen in Figure 8.
Figure 7: Tulle Material