Laboratory Team: IDEXX

SaniPath Daily Laboratory List

· Distilled water for dilutions and to make PBS

· Alcohol for sterilization

· PBS for

o All of the dilutions

o Processing Soil and street food

· PBST for…

o Processing of Vegetables

o Recipe: 1L of normal PBS, and 0.5mL of Tween-80

· Serological Pipettes (10mL) for all samples

· Micropipette (1mL) for all samples

· Graduated cylinders (100mL and 500mL) for all samples

· 120mL stand up Whirl-Pak bags for final dilutions and mixing Colilert

· Sterile conical tubes (15mL) for dilutions

· Sterile conical tubes (50mL) for soil samples

· Weigh scale for produce, street food, and soil samples

· Vortex for swab samples

· Shaker for soil samples

· pH strips for soil

· IDEXX equipment

o IDEXX sealer

o IDEXX Tray

o Colilert-24

· Android device for data entry

If the laboratory does not have any of these items please contact laboratory/project manager and let him/her know immediately.

IDEXX: Did you remember?

· To put a total volume of a 100mLs in the IDEXX tray?

· To make sure you do not touch the inside of the sample tray?

· To make sure the trays do not have bubbles?

· To put your sample tray in the red colored rubber Quantitray before inserting it in the sealer? (Do not use the gray one)

· To insert the quantitray with your sample into the sealer with the filled cells facing down?

· To run a negative control?

Yes? Great work!