Supplies and Daily Laboratory Lists

SaniPath Supplies Checklist

Sample Collection

· Blank Adhesive Labels:

· Sterile Bucket:

· Spray Bottles:

· Framing Square:

· Pens, Permanent Markers:

· Android Devices:

· Batteries for Androids:

· Spatula:

· Large Ice Chest:

· Small Ice Chest:

· Ice Packs:

· 100 mL Whirl-Pak Bags w/ Sodium Thiosulfate:

· 100 mL Whirl-Pak Bags:

· 2 L Whirl-Pak Bags:

· Chlorine Test Kit w/ Supplies:

· Cotton/Paper Towels:

· EnviroMax Swabs:

· Gloves:

General Laboratory Supplies

· Incubator:

· Refrigerator:

· Autoclave:

· Gloves:

· Racks for Whirl-Paks:

· 90% Ethanol (mL):

· 70% Ethanol (mL):

· Alcohol Burner:

· Pipette Tips:

· Pipette-Aid:

· 500 mL Gradauted Cylinder:

· 100 mL Beaker:

· PBS:

· PBS w/ Tween:

· Sterile Water:

· Vortex:

· Shaker:

· Scale:

· 5 or 10 mL Serological Pippetes*:

· 15 mL Conical Tube:

· 50 mL Conical Tube:

· Pipette/Dropper:

· NaOH:

· pH Strips:

· 10 mL Pasteur Pipette:

IDEXX Supplies

· IDEXX Quanti-Tray Sealer:

· Colilert:

· IDEXX quanti-trays:

Membrane Filtration Supplies

· Rubber Tubing:

· 1 L Side Arm Flask:

· Membrane Filter Holder & Funnel:

· Vacuum Pump:

· Vacuum Manifold:

· Membrane Filters:

· Filter Pad:

· m-Coli Blue or Bio-Rad culture media:

Daily Laboratory List: IDEXX

· Distilled water for dilutions and to make PBS

· Alcohol for sterilization

· PBS for

- All of the dilutions

- Processing Soil and street food

· PBST for…

- Processing of Vegetables

- Recipe: 1L of normal PBS, and 0.5mL of Tween-80

· Serological Pipettes (10mL) for all samples

· Micropipette (1mL) for all samples

· Graduated cylinders (100mL and 500mL) for all samples

· 120mL stand up Whirl-Pak bags for final dilutions and mixing Colilert

· Sterile conical tubes (15mL) for dilutions

· Sterile conical tubes (50mL) for soil samples

· Weigh scale for produce, street food, and soil samples

· Vortex for swab samples

· Shaker for soil samples

· pH strips for soil

· IDEXX equipment

- IDEXX sealer

- IDEXX Tray

- Colilert-24

· Android device for data entry

If the laboratory does not have any of these items please contact laboratory/project manager and let him/her know immediately.

Daily Laboratory List: Membrane Filtration

· Distilled water to...

- Make PBS

- Rinse filter holder during filtration

· Alcohol for sterilization

· PBS for

- All of the dilutions

- Processing Soil

· PBST for…

- Processing of Vegetables

- Recipe: 1L of normal PBS, and 0.5mL of Tween-80

· Pipettes (10mL, 1mL) for all samples

· Graduated cylinders (100mL and 500mL) for all samples

· Sterile conical tubes (15mL) for dilutions

· pH strips for soil

· Filtration equipment

- Prepared plates with BioRad media

- Sterile filters

- Alcohol burner

- Sterile Forceps

If the laboratory does not have any of these items please contact laboratory/project manager and let him/her know immediately.