羅竹芳 (Lo, Chu-Fang)
國立成功大學 生物科技與產業科學系 名譽講座教授
E-Mail: gracelow@mail.ncku.edu.tw
學歷：
日本東京大學水產學科 博士
國立臺灣大學動物學系 碩士
輔仁大學生物系 學士
經歷
國立成功大學生物科學與科技學院 院長
國立臺灣大學生命科學院院 院長
國立臺灣大學動物學研究所 特聘教授
國立臺灣大學系統生物學中心 主任
國立成功大學生物科技與產業科學系 講座教授
國立成功大學前瞻蝦類養殖國際研發中心 主任
行政院科技會報辦公室 兼任研究員
現職
成大研究發展基金會/特聘研究員
國立成功大學 生物科技與產業科學系 名譽講座教授
教育部生物醫農領域 終身國家講座
世界動物衛生組織 WSD參考實驗室 主持人兼專家
世界動物衛生組織 AHPND參考實驗室 主持人兼專家
研究榮譽獎項
中國生物學會傑出生物科學研究獎 (1999)
行政院國家科學委員會傑出研究獎 (1997-2000)
行政院國家科學委員會傑出研究獎 (2000-2003)
行政院傑出科技榮譽獎 (2001)
經典引文獎ISI® Citation Classic Award (2001) in recognition of high impact research for the highly cited paper: Detection of baculovirus associated with white spot syndrome (WSBV) in penaeid shrimps using polymerase chain reaction. (published 1996)
行政院國家科學委員會92年度傑出技術移轉貢獻獎 (2003)
行政院國家科學委員會傑出研究獎 (2003-2006)
教育部第51屆生物及醫農科學類科學術獎 (2007)
教育部第12屆生物及醫農科學類科國家講座獎 (2008-2011)
第五屆「台灣傑出女科學家獎」(2012)
教育部第20屆生物及醫農科學類科國家講座(2016-2019；終身國家講座)
第26屆東元獎(科技類) (2019)
研究歷程
當1994年我接到第一批罹患蝦白點症的蝦樣本時，雖然未曾有從事甲殼動物的相關研究經驗，不過本人於1986年到美國研習的桿狀病毒/昆蟲細胞真核表現載體系統的經驗，讓我能立即切入探究這一重挫全球經濟的養殖蝦類新興疾病。當時我很快就解決了蝦體黏液及DNA分解酵素干擾的問題，成功地純化出致病病毒。同時在三年內即發表4篇ISI高引用論文。我的研究由從初期研究水生無脊椎動物病毒性疾病(白點症)爆發的特性及機制開始，再而深入研究致病原的基因體及分子特性，最近則專研蝦的防禦機制和宿主-病原體之交互作用等。研究成果促使1. 全球對蝦疾病、海洋病毒及其他病原體的獨特性的重視；2.養蝦產業(苗場及養成場)生物安全上的運作的改革；3. 蝦白點症致病原-白點症病毒成為新病毒科Nimaviridae的模式物種；4.海洋無脊椎動物與病原互動及的防禦機制的研究受到學術界重視。
本人前瞻研究工作並不侷限於病毒本身，目前已延伸至致病機制和防疫措施的探究。最近本人除了繼續研究宿主-病毒交互作用外，亦從事另一重要快速擴散的蝦類新興疾病-急性肝胰腺壞死病(Acute hepatopancreatic necrosis disease, AHPND)。急性肝胰腺壞死病的致病原為海洋副溶血孤菌(Vibrio parahaemolyticus)，相關研究成果發表在 “美國國家科學院院刊”，闡釋這種常見的海洋伺機性細菌因新獲得一個攜帶致死毒素基因之質體，進而轉變成毒力強大的病原體。
本人及團隊在蝦類疾病的突破性基礎研究成果及前瞻疾病控制技術在蝦類養殖產業上的應用，備受國內及國際肯定。目前本人實驗室是世界動物衛生組識(OIE)之蝦白點症(WSD)及急性肝胰腺壞死症(AHPND)參考實驗室。25年間共發表160篇以上的期刊論文，總引用次數超過8,662次(h-index: 50)，並且在國際會議受邀演溝達四十次以上。因研究表現受到肯定而使本人榮獲許多重要獎項：例如國科會傑出研究獎、教育部學術獎、教育部終生國家講座、吳健雄基金會傑出女科學家獎及東元獎。
在中心任務
代表團隊應邀在國際會議發表中心成果
主持世界動物衛生組識兩種蝦類疾病參考實驗室
參與全球養殖蝦疫病防控任務，成就獲世界動物衛生組織肯定而頒予OIE 2020 Meritorious Award (我國第一位獲頒此獎)
2016年進駐本中心成大安南校區實驗站建立草蝦育種技術平台
2020年進駐本中心成大花蓮實驗站，建立生產商用草蝦種蝦技術平台
期刊論文 (76 papers selected from 161 papers *corresponding author)
Lin SJ, Huang JY, Le PT, Lee CT, Chang CC, Yang YY, Su Y, Lo CF*, Wang HC. (2022). Expression of the AHPND Toxins PirAvp and PirBvp Is Regulated by Components of the Vibrio parahaemolyticus Quorum Sensing (QS) System. Int. J. Mol. Sci. 2022, 23, 2889. IF: 5.924; Ranking 22% (67/295).
Huang JY, Wang HC, Chen YC, Wang PS, Lin SJ, Chang YS, Liu KF and Lo CF*. (2020) A shrimp glycosylase protein, PmENGase, interacts with WSSV envelope protein VP41B and is involved in WSSV pathogenesis. Dev. Comp. Immunol. 108. 103667. IF: 3.119; Ranking 4.1% (7/170).
Apitanyasai, K., Chang, C.C., Ng, T.H., Ng, Y.S., Liou, J.H., Lo, C.F., Lin, S.S., Wang, H.C. (2020) Penaeus vannamei serine proteinase inhibitor 7 (LvSerpin7) acts as an immune brake by regulating the proPO system in AHPND-affected shrimp. Dev. Comp. Immunol. 106:103600. IF: 3.119; Ranking 4.1% (7/170).
Kumar, R., Ng, TH., Chang, C.C., Tung, T.C., Lin, S.S., Lo, C.F., Wang, H.C. (2020) Bile acid and bile acid transporters are involved in the pathogenesis of acute hepatopancreatic necrosis disease in white shrimp Litopenaeus vannamei. Cell Microbiol. 22:e13127. IF: 4.288; Ranking: 23.31 (31/133)
Apitanyasai, K., Huang, S.W., Ng, T.H., He, S.T., Huang, Y.H., Chiu, S.P., Tseng, K.C., Lin, S.S., Chang, W.C., Baldwin-Brown, J.G., Long, A.D., Lo, C.F., Yu, H.T., Wang, H.C. (2019) The gene structure and hypervariability of the complete Penaeus monodon Dscam gene. Sci Rep. 9:16595. IF: 4.011; Ranking: 21.74% (15/69).
Jaree, P., Kawai, T., Lo, C.F., Tassanakajon, A., Somboonwiwat, K*. (2019) Genome organization and definition of the Penaeus monodon viral responsive protein 15 (PmVRP15) promoter. Fish and Shellfish Immunology 93: 997-1006. IF: 3.298; Ranking: 2.13% (3/141).
Lin, S.J., Chen, Y.F., Hsu, K.C., Chen, Y.L., Ko, T.P., Lo, C.F., Wang, H.C., Wang, H.C*. (2019) Structural insights to the Heterotetrameric Interaction between the Vibrio parahaemolyticus PirAvp and PirBvp Toxins and Activation of the Cry-Like Pore-Forming Domain. Toxins 11:233. IF: 3.895; Ranking: 14.07% (19/135)
Ng, T.H., Lu, C.W., Lin, S.S., Chang, C.C., Tran, L.H., Chang, W.C., Lo, C.F., Wang, H.C*. (2018) The Rho signaling pathway mediates the pathogenicity of AHPND-causing V. parahaemolyticus in shrimp. Cell Microbiol. 6: e12849. IF: 4.288; Ranking: 23.31 (31/133)
Kumar, R., Chang, C.C., Ng, T.H., Ding, J.Y., Tseng, T.C., Lo, C.F., Wang, H.C. (2018) Draft Genome Sequence of Vibrio parahaemolyticus Strain M1-1, Which Causes Acute Hepatopancreatic Necrosis Disease in Shrimp in Vietnam. Genome Announc. 6(3): e01468-17. Time cited: 2.
Sun, C.K*., Tsai, Y.C., Chen, Y.E., Liu, T.M., Chen, H.Y., Wang, H.C., Lo, C.F. (2017) Resonant dipolar coupling of microwaves with confined acoustic vibrations in a rod-shaped virus. Sci. Rep. 7(1):4611. IF: 4.011; Ranking: 21.74% (15/69).
Huang, J.Y., Kang, S.T., Chen, I.T., Chang, L.K., Lin, S.S., Kou, G.H., Chu, C.Y., Lo, C.F.* (2017) Shrimp miR-10a is co-opted by white spot syndrome virus to increase viral gene expression and viral replication. Front. Immunol. 8:1084. IF: 4.716; Ranking: 27.22% (43/158).
Ponprateep, S., Vatanavicharn, T., Lo, C.F., Tassanakajon, A., Rimphanitchayakit, V. (2016) Alpha-2-macroglobulin is a modulator of prophenoloxidase system in pacific white shrimp Litopenaeus vannamai. Fish Shellfish Immunol. 62:68-74. IF: 3.298; Ranking: 2.13% (3/141).
Chen, I.T., Lee, D.Y., Huang, Y.T., Kou, G.H., Wang, H.C., Chang, G.D., Lo, C.F.* (2016) Six hours after infection, the metabolic changes induced by WSSV neutralize the host's oxidative stress defenses. Sci. Rep. 6:27732. IF: 4.011; Ranking: 21.74% (15/69).
Liu, W.J., Li, Y.C., Kou, G.H., Lo, C.F.* (2016) Laminin receptor in shrimp is a cellular attachment receptor for white spot syndrome virus. PLoS One 11(6), e0156375. IF: 2.776; Ranking: 34.78% (24/69).
Jaree, P., Senapin, S., Hirono, I., Lo, C.F., Tassanakajon, A., Somboonwiwat, K. (2016) WSV399, a viral tegument protein, interacts with the shrimp protein PmVRP15 to facilitate viral trafficking and assembly. Dev. Comp. Immunol. 59, 177-185. IF: 3.119; Ranking: 4.12% (7/170).
Lai, H.C., Ng, T.H., Ando, M., Lee, C.T., Chen, I.T., Chuang, J.C., Mavichak, R., Chang, S.H., Yeh, M.D., Chiang, Y.A., Takeyama, H., Hamaguchi, H.O., Lo, C.F., Aoki, T., Wang, H.C. (2015) Pathogenesis of acute hepatopancreatic necrosis disease (AHPND) in shrimp. Fish Shellfish Immunol. 47(2), 1006-1014. IF: 3.298; Ranking: 2.13% (3/141).
Lee, C. T., Chen, I. T., Yang, Y. T., Ko, T. P., Huang, Y. T., Huang, J. Y., Huang, M. F., Lin, S. J., Chen, C. Y., Lin, S. S., Lightner D. V., Wang, H. C., Wang A. H., Wang, H. C.*, Hor, L. I.*, Lo, C. F.* (2015). The opportunistic marine pathogen Vibrio parahaemolyticus becomes virulent by acquiring a plasmid that expresses a deadly toxin. Proc Natl Acad Sci U S A. 112(34), 10798-10803. IF: 9.58; Ranking: 10.14% (7/69).
Leu, J. H.*, Liu, K. F., Chen, K. Y., Chen, S. H., Wang, Y. B., Lin, C. Y., Lo, C. F. (2015). The novel white spot syndrome virus-induced gene, PmERP15, encodes an ER stress-responsive protein in black tiger shrimp, Penaeus monodon. Dev Comp Immunol. 49(2), 239-248. IF: 3.119; Ranking: 4.12% (7/170).
Lin, S. J., Lee, D. Y., Wang, H. C., Kang, S. T., Hwang, P. P., Kou, G. H., Huang, M. F., Chang, G. D.*, Lo, C. F.* (2015). White spot syndrome virus protein kinase 1 (PK1) defeats the host cell's Iron withholding defense mechanism by interacting with host ferritin. J Virol. 89(2), 1083-1093. Lin, S.J., Lee, D.Y., Wang, H.C., Kang, S.T., Hwang, P.P., Kou, G.H., Huang, M.F., Chang, G.D.*, Lo, C.F.* (2015). White spot syndrome virus protein kinase 1 defeats the host cell's iron-withholding defense mechanism by interacting with host ferritin. J Virol. 89(2), 1083-1093. IF: 4.324; Ranking: 22.22% (8/36).
Yang, Y. T., Chen, I. T., Lee, C. T., Chen, C. Y., Lin, S. S., Hor, L. I., Tseng, T. C., Huang, Y. T., Sritunyalucksana K., Thitamadee S., Wang, H. C., Lo, C. F.* (2014). Draft genome sequences of four strains of Vibrio parahaemolyticus, three of which cause early mortality syndrome/acute hepatopancreatic necrosis disease in shrimp in China and Thailand. Genome Announc. 2(5), e00816-14.
Yang, Y. T., Lee, D. Y., Wang, Y., Hu, J. M., Li, W. H., Leu, J. H., Chang, G. D., Ke, H. M., Kang, S. T., Lin, S. S., Kou, G. H.*, Lo, C. F.* (2014). The genome and occlusion bodies of marine Penaeus monodon nudivirus (PmNV, also known as MBV and PemoNPV) suggest that it should be assigned to a new nudivirus genus that is distinct from the terrestrial nudiviruses. BMC Genomics. 15(628), 1471-2164. IF: 3.501; Ranking: 29.63% (48/162).
Su, M. A., Huang, Y. T., Chen, I. T., Lee, D. Y., Hsieh, Y. C., Li, C. Y., Ng, T. H., Liang, S. Y., Lin, S. Y., Huang, S. W., Chiang, Y. A., Yu, H. T., Khoo, K. H., Chang, G. D., Lo, C. F, Wang, H. C.* (2014). An invertebrate warburg effect: a shrimp virus achieves successful replication by altering the host metabolome via the PI3K-Akt-mTOR pathway. PLoS Pathog. 10(6), e1004196. IF: 6.463; Ranking: 8.33% (3/36).
Sangsuriya P., Huang, J. Y., Chu, Y. F., Phiwsaiya K., Leekitcharoenphon P., Meemetta W., Senapin S., Huang, W. P., Withyachumnarnkul B., Flegel, T. W.*, Lo, C. F.* (2014). Construction and application of a protein Interaction map for white spot syndrome virus (WSSV). Mol Cell Proteomics. 13(1), 269-282. IF: 4.828; Ranking: 8.86% (7/79).
Kang, S. T., Wang, H. C., Yang, Y. T., Kou, G. H.*, Lo, C. F.* (2013). The DNA virus WSSV uses an internal ribosome entry site (IRES) for translation of the highly expressed non-structural protein ICP35. J Virol. 87(24), 13263-13278. IF: 4.324; Ranking: 22.22% (8/36).
Leu, J. H.*, Lin, S. J., Huang, J. Y., Chen, T. C., Lo, C. F. (2013). A model for apoptotic interaction between white spot syndrome virus and shrimp. Fish Shellfish Immunol. 34(4), 1011-1017.
Huang, J. Y., Liu, W. J., Wang, H.C., Lee, D. Y., Leu, J. H., Wang, H. C., Tsai, M. H., Kang, S. T., Chen, I. T., Kou, G. H., Chang, G. D.*, Lo, C. F.* (2012). Penaeus monodon thioredoxin restores the DNA binding activity of oxidized WSSV IE1. Antioxid Redox Signal. 17(6) 914-926.
Lin, S. J., Hsia, H. L., Liu, W. J., Huang, J. Y., Liu, K. F., Chen, W. Y., Yeh, Y. C., Huang, Y. T., Lo, C. F., Kou, G. H.*, Wang, H. C.* (2012). Spawning stress triggers WSSV replication in brooders via the activation of shrimp STAT. Dev. Comp. Immunol. 38(1), 128-135.
Chang, L. K., Huang, P. H., Shen, W. T., Yang, S. H., Liu, W. J., Lo, C. F.* (2012). Role of Penaeus monodon Kruppel-like factor (PmKLF) in infection by white spot syndrome virus. Dev. Comp. Immunol. 36 (1), 121-129.
Huang, S. W., Lin, Y. Y., You, E. M., Liu, T. T., Shu, H. Y., Wu, K. M., Tsai, S. F., Lo, C. F., Kou, G. H., Ma, G. C., Chen, M., Wu, D., Aoki, T., Ikuo H., Yu H. T.* (2011). Fosmid library end sequencing reveals a rarely known genome structure of marine shrimp Penaeus monodon. BMC Genomics. (12)242.
Chen, I. T, Aoki, T., Huang, Y. T., Hirono, I., Chen, T. C., Huang, J. Y., Chang, G. D., Lo, C. F., Wang, KC. H. C.* (2011). White spot syndrome virus induces metabolic changes resembling the Warburg effect in shrimp hemocytes in the early stage of infection. J Virol. 85(24)12919-12928.
Liu, W. J., Chang, Y. S., Huang, W. T., Chen, I. T., Wang, K. C., Kou, G. H.*, Lo, C. F.* (2011) Penaeus monodon TATA box-binding protein (PmTBP) interacts with the WSSV transactivator IE1 and promotes its transcriptional activity. J Virol. 85(13), 6535-6547.
Lin, Y. R., Hung, H. C., Leu, J. H., Wang, H. C., Kou, G. H.*, Lo, C. F.* (2011). The role of ALDH and HSP70 in the suppression of white spot syndrome virus replication at high temperature. J Virol. 85(7), 3517-3525.
Chang, Y. S., Chen, T. C., Liu, W. J., Kou, G. H.*, Lo, C. F.*, (2011). Assessment of the roles of copepod Apocyclops royi and bivalve mollusk Meretrix lusoria in white spot syndrome virus transmission. Mar Biotechnol.13(5), 909-917.
Nupan B, Phongdara A*, Saengsakda M, Leu, J. H., Lo, C. F. (2011). Shrimp Pm-fortilin inhibits the expression of early and late genes of white spot syndrome virus (WSSV) in an insect cell model. Dev Comp Immunol. 35(4) 469-475.
Leu, J. H., Chen, S. H., Wang, Y. B., Chen, Y. C., Su, S. Y., Lin, C. Y.*, Ho, J. M.*, Lo, C. F.* (2011). A review of the major penaeid shrimp EST studies and the construction of a shrimp transcriptome database based on the ESTs from four penaeid shrimp. Mar Biotechnol. 13(4), 608-621.
Leu, J. H., Chen, L. L., Lin, Y. R., Kou, G. H., Lo, C. F.* (2010). Molecular mechanism of the interactions between white spot syndrome virus anti-apoptosis protein AAP-1 (WSSV449) and shrimp effector caspase. Dev Comp Immunol. 34(10) 1068-1074.
Chang, Y. S., Liu, W. J., Lee, C. C., Chou, T. L., Lee, Y. T., Wu, T. S., Huang, J. Y., Huang, W. T., Lee, T. L., Kou, G. H., Wang, A. H. J.*, Lo. C. F.* (2010). A 3D model of the membrane protein complex formed by the white spot syndrome virus structural proteins. PLoS One. 5(5): e10718.
Liu, K. F., Yeh M. S., Kou, G. H., Cheng, W.*, Lo, C. F.* (2010). Identification and cloning of a selenium-dependent glutathione peroxidase from tiger shrimp, Penaeus monodon, and its transcription following pathogen infection and related to the molt stages. Dev Comp Immunol. 34(9) 935-944.
Liu, K. F., Liu, W. J., Kou, G. H., Lo. C. F.* (2009). Shrimp white spot syndrome – from pathology to pathogenomics. Fish Pathol. 44(2), 55-58.
Kang, S. T., Leu , J. H., Wang, H. C., Chen, L. L., Kou, G. H.*, Lo. C. F.* (2009). Polycistronic mRNAs and internal ribosome entry site elements (IRES) are widely used by white spot syndrome virus (WSSV) structural protein genes. Virology. 387(2),353-63.
Wang, H. C., Wang, H. C., Ko, T. P., Lee, Y. M., Leu, J. H. , Ho, C. H. ,Huang, W. P. Lo, C. F.*, Wang, A.H-J.* (2008). White spot syndrome virus protein ICP11: A histone-binding DNA mimic that disrupts nucleosome assembly. Proc. Natl. Acad. Sci. USA. 105(52), 20758-20763.
Chang, Y. S.*, Liu, W. J., Chou, T. L., Lee, Y. T., Lee, T. L., Huang, W. T., Kou, G, H, Lo, C. F.* (2008). Characterization of white spot syndrome virus envelope protein VP51A and its interaction with viral tegument protein VP26. J Virol. 82(24), 12555-12564.
Liu, W. J., Chang, Y. S., Wang, H. C., Leu, J. H., Kou, G, H.*, Lo, C. F.* (2008). Transactivation, dimerization, and DNA-binding activity of white spot syndrome virus immediate early protein IE1. J Virol. 82(22), 11362-11373.
Chen, W. Y., Hoa, K. C., Leu, J. H., Liu, K. F., Wang, H. C., Kou, G. H.*, Lo, C. F.* (2008). WSSV infection activates STAT in shrimp. Dev. Comp. Immunol. 32(10), 1142-1150.
Leu, J. H., Wang, H. C., Kou, G. H.*, Lo, C. F.* (2008). Penaeus monodon caspase is targeted by a white spot syndrome virus anti-apoptosis protein. Dev. Comp. Immunol. 32(5), 476-86.
Leu, J. H., Kuo, Y. C., Kou, G. H.*, Lo, C. F.*, (2008). Molecular cloning and characterization of an inhibitor of apoptosis protein (IAP) from the tiger shrimp, Penaeus monodon. Dev Comp Immunol. 32(2), 121-33.
Rattanarojpong, T., Wang, H. C., Lo, C. F.*, Flegel, T. W. (2007). Analysis of differently expressed proteins and transcripts in gills of Penaeus vannamei after yellow head virus infection. Proteomics. 7(20), 3809-3814.
Leu, J. H., Chang, C. C., Wu, J. L., Hsu, C. W., Hirono Ikuo, Aoki, T., Juan H. F., Lo, C. F., Kou, G. H.*, Huang H. C.* (2007). Comparative analysis of differentially expressed genes in normal and white spot syndrome virus infected Penaeus monodon. BMC Genomics. (8)120, 1471-2164.
Wang, H. C., Wang, H. C., Leu, J. H., Kou, G. H., Wang, A.H-J.*, Lo, C. F.*, (2007). Protein expression profiling of the shrimp cellular response to white spot syndrome virus infection. Dev Comp Immunol. 31,672-686.
Liu, W. J., Chang, Y. S., Wang, A.H-J., Kou, G. H.*, Lo, C. F.*, (2007). White spot syndrome virus annexes a shrimp STAT yo enhance expression of the immediate-early gene ie1. J Virol. 81(3), 1461-1471.
Sritunyalucksana, K., Wannapapho, W., Lo, C. F., Flegel, T. W.*, (2006). PmRab7 is a VP28-binding protein involved in white spot syndrome virus (WSSV) infection in shrimp. J Virol. 80, 10734-10742.
Tsai, J. M., Wang, H. C., Leu, J. H., Wang, A.H-J., Zhuang,Y., Walker, P. J., Kou, G. H.*, Lo, C. F.* (2006). Identification of the nucleocapsid, tegument and envelope proteins of the shrimp white spot syndrome virus virion. J Virol. 80(6), 3021-3029.
Liu, W. J., Chang, Y. S., Wang, C. H., Kou, G. H.*, Lo, C. F.*, (2005). Microarray and RT-PCR screening for white spot syndrome virus immediate-early genes in cycloheximide-treated shrimp. Virology. 334, 327-341.
Leu, J. H., Tsai, J. M., Wang, H. C., Wang, A.H-J., Wang, C. H., Kou, G. H.*, Lo, C. F.* (2005). The unique stacked rings in the nucleocapsid of the WSSV virion are formed by the major structural protein VP664, the largest viral structural protein ever found. J Virol. 79, 140-149.
Lo, C. F.*, Peng, S. E., Chang, Y.S., Kou, G.H. (2005). White spot syndrome – What we have learned about the virus and the disease. In P. Walker, R. Lester and M.G. Bondad-Reantaso (eds). Diseases in Asian Aquaculture V. pp. 421-433.
Wang, H. C., Chang, Y. S., Kou, G. H., Lo, C. F.* (2004). White spot syndrome virus: molecular characterization of a major structural protein in a baculovirus expression system and shrimp hemocytes. Mar. Biotechnol. 6, S95-99.
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