TROSY-PFG

2D [1H, 15N]-TROSY.

[Narrow and wide bars stand for non-selective 90 and 180. radio-frequency pulses along the H and N channel, with delay tau= 2.7ms.PFG indicates the pulsed magnetic field gradients applied along z-axis; G is gradient amplitude.Water saturation is minimized by the application of the rf-pulses along the H channel indicated by curved shapes]

Cara Molecular Viewer

Fid 1 from the 2D TROSY of Ubiquitin

References

PDF Pervushin, K., R. Riek, G. Wider, and K. Wuthrich, Attenuated T2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution. Proceedings of the National Academy of Sciences of the United States of America, 1997. 94(23): p. 12366-71.

PDF Pervushin, K. V., G. Wider, and K. Wuthrich, Single Transition-to-single Transition Polarization Transfer (ST2-PT) in [15N,1H]-TROSY. Journal of biomolecular NMR, 1998. 12(2): p. 345-8.