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For backbone resonance assignment, 15N, 13C-labeled protein is used to record 3D NMR experiments such as HNCA, HNCACB, CBCA(CO)NH and HN(CO)CA.
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For backbone resonance assignment, 15N, 13C-labeled protein is used to record 3D NMR experiments such as HNCA, HNCACB, CBCA(CO)NH and HN(CO)CA.