HC cross polarization

Experiment Details

Dimensions = 1

Isotope labeling required = ¹³N

Magnetization transfer = ¹H - ¹³C

Pulse program: hC.cp (basic cross polarization experiment)

Parameters:

F1, C and F2, H

p1 : C 90 at pl1(acqt0-calculation & -DC90)

p3 : H 90 at pl2

p15 : HC CP at sp41 (f1,C) and sp40 (f2,H)

pl1 : C pulse power

pl2 : H pulse power

pl12 : H dec power

sp40 : H CP power

sp41 : C CP power

d1 : recycle delay; 1 to 5 times T1

pcpd2 : pulse length in decoupling sequence cpdprg2

cpdprg2 : spinal64, swftppm, tppm etc. decoupling program

spnam40 : H ramp use e.g. ramp.10070 for variable amplitude CP

spnam41 : C ramp use e.g. square.100 for square pulse CP

ZGOPTNS : -DC90 : optimize C 90 power

-Dlacq : aq is longer than 50 ms or blank

Comment on key optimization:

ZGOPTNS = '-DC90' for C 90 cal

Example spectrum of GB1: Recorded at MAS 17857 Hz in a 3.2 mm rotor.