ccDREAM

Experiment Details

Dimensions = 2

Isotope labeling required = ¹³C

Magnetization transfer = polarization is transferred between Ca and Cb and aliphatic side-chain carbons.

Pulse program: hCC.cp

J Biomol NMR (2012) 53:103-112

Parameters:

In spectra recorded at 13 kHz, the SPINAL64 scheme was used.

In spectra recorded at 24kHz, the XiX scheme was used during t1 and t2 with rf field strength of 100kHz.

cw-decoupling: 100kHz

mixing time: 5 ms

in spectra recorded at 24kHz, the center amp of the DREAM sweep was 11.5kHz, the big delta of the sweep 3 kHz and the estimated dipolar coupling 1 kHz.

in spectra recorded at 13kHz, the center amp of the DREAM sweep was 6 kHz, the big delta of the sweep 2 kHz and the estimated dipolar coupling 1 kHz.

1024 points with 16 scans in the indirect dimension were acquired using TPPI.

Example spectrum: J Biomol NMR (2012) 53:103-112

U-( 13 C, 15 N) Ubiquitin were acquired on a Bruker AV600 spectrometer operating at a static field of 14.09 T corresponding to a proton Larmor frequency of 600 MHz. A Bruker 3.2 mm triple-resonance probe was used. MAS was actively stabilized to within ±10 Hz. The temperature of the sample was stabilized at about 3°C.

References:

1. J Biomol NMR (2012) 53:103-112

2. JMR 150, 81-99 (2001)

3. JMR 142, 97-101 (2000)

4. A.Detken et al./ Chemical Physics Letters 356 (2002) 298–304