HN cross polarization

Experiment Details

Dimensions = 1

Isotope labeling required = ¹⁵N

Magnetization transfer = ¹H - ¹⁵N

Pulse program: hN.cp (basic cross polarization experiment)

Parameters:

F1,N and F2, H

p3: H 90 at pl2

p25: HN CP at sp43 (f1,N) and sp42 (f2,H)

plw2: H pulse power

plw12: H dec power

sp42: H CP power

sp43: N CP power

d1: recycle delay, 1 to 5 times T1

pcpd2: pulse length in decoupling sequence cpdprg2

cpdprg2: spinal64, swftppm, tppm etc. decoupling program

spnam42: H ramp use e.g. ramp.10070 for variable amplitude CP

spnam43: N ramp use e.g. square.100 for square pulse CP

ZGOPTNS: -Dlacq : aq is longer than 50 ms

-DN90 : optimize N 90 power or blank

Comment on key optimization:

ZGOPTNS = '-DC90' for N 90 cal

Example spectrum of GB1: