hsqc-pfg

Fig3 2D H-N HSQC with PFG recorded on below sample#

#Sample: 0.02mM, 15N, 13C-labeled Ubiquitin in 20mM HEPES at pH 6.8 with 10% D2O

PULSE SEQUENCE DESCRIPTION (BRIEF)

1n mid 90's, there was already advancement of pulse field gradients and incorporation into NMR pulse sequences for high resolution NMR. Lewis key described in their JACS paper, about the use of a pulse sequence for pure absorption HSQC with coherence transfer selection achieved using gradients.

REFERENCES

1. Kay et al.,1992, JACS, Pure Absorption Gradient Enhanced HSQC with improved sensitivity, 114,26.