Fixed tissue will be infiltrated with our unique tissue cryoprotection solution (cf. Products, Cat. #NDT302) and then rapidly frozen in isopentane precooled to -70°C. The frozen tissue will be stored at -75°C before sectioning.
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Following a thorough wash in distilled water, fixed tissue will be dehydrated through graded ethanol, cleared in xylenes, and then impregnated and embedded in paraffin.
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Please contact us for more information.