FD Apop Kit

NDT101 FD Apop^TM Kit

• Price: $571 (For 50 sections)

This kit is designed for the microscopic detection of cells undergoing apoptosis based on the principle of in situ DNA nick-end labeling (TUNEL) technique¹. The assay uses terminal deoxynucleotidyl transferase to catalyze the incorporation of biotinylated deoxyuridines onto the free 3'-hydroxyl termini of DNA fragments, which are considered as one of the most characteristic features of apoptosis^2,3. The integrated biotins are amplified and visualized with the avidin-biotin-complex (ABC) method⁴, enabling light microscopic identification⁵.

The reagents and procedure of the Kit have been optimized to achieve a high degree of both specificity and sensitivity for detecting apoptotic cells with minimal background. This kit can be used with frozen and paraffin sections, as well as cultured cells. The procedure of the kit takes approximately 4 hours.

Kit Contents:

Part I (Store at -20^oC):

• Reaction Solution A (2 ml x 2)
• Reaction Solution B (60 ul)
• Reaction Solution C (40 ul)
• Chromogen Solution (20 ml)

Part II (Store at 4^oC):

• Equilibration Buffer (20 ml)
• Detection Reagent (6 ml)
• 10x Phosphate-Buffered Saline (250 ml x 2)

Materials required, but not included:

• Double distilled water
• Humidified chamber
• Incubator or waterbath (30^oC)
• Histological supplies and equipment, including microscope slides, glass coverslips, staining jars, fine-tipped forceps, ethanol, xylenes or xylene-substitutes, mounting medium, and a light microscope.

References:

1. Gavrieli Y., Sherman Y. and Ben-Sasson S. A. (1992) Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell Biol. 119: 493-501.
2. Wyllie A. H. (1980) Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature 284: 555-556.
3. Arends M. J., Morris R. G. and Wyllie A. H. (1990) Apoptosis: the role of the endonuclease. Amer. J. Pathol. 136: 593-608.
4. Hsu S. M., Raine L. and Fanger H. (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29: 577-580.
5. Giovana R. Thomas, Zhong Chen, Ileana Enamorado, Caren Bancroft, Carter Van Waes (2000) IL-12- and IL-2-induced tumor regression in a new murine model of oral squamous-cell carcinoma is promoted by expression of the CD80 co-stimulatory molecule and interferon. Int. J. Cancer: 86, 368-374.

Terms and Conditions

• For quality assurance of our service, it is recommended that you discuss with us for preferred perfusion protocol and histology and/or immunolabeling protocols.
• It is suggested that you use Gel-coated microscopic slides for tissue mounting and 0.17um-thick coverslips.
• A 15% of the fee will be due upon authorization of the study; and the remaining fee will be due upon delivery of study results.
• Progress of the service is contingent upon staining quality of tissues, operated by the independent contractor.
• Should early termination occur, Neurodigitech will prorate the cost incurred and invoice the Sponsor. The first portion of the fee is non-refundable.