GolgiChrome staining Service

GolgiChrome^TM staining Service (Double-stainiing of Golgi impregnation and immunohistochemistry)

This invention is designed to achieve the simultaneous visualization of Golgi-Cox and immunofluorescence of individual neurons using Confocal Laser Scanning Microscopy (CLSM).

The main advantages of this method are as follows:

• Highest visualization of structural details along with the neurochemical nature of the neuron;
• Characterization of antigens with specific antibodies
• Anatomical interactions among neuronal elements;
• The possibility of using confocal laser scanning microscope (CLSM) and accordingly 3D reconstructions and modeling;
• Savings of laboratory animals, when up to now it was necessary to perform the two techniques separately and thus on different animals.
• Consequent substantial advantages in terms of time and cost saving, together with a higher quality than traditional techniques.

The following examples (Spiga et al., 2012) demonstrate the co-localizations of Golgi-cox impregnated neurons immunoreactive to Tyrosine Hydroxylase (TH) (Figure 1), TH and Postsynaptic Density 95 (PSD-95) (Figure 2), TH, PSD-95 or Synapsin (SynI) (Figure 3), and TH, PSD-95 or SynI of a fully impregnated pyramidal cell (Figure 4).

Terms and Conditions

• For quality assurance of our service, it is recommended that you discuss with us for preferred perfusion protocol and histology and/or immunolabeling protocols.
• It is suggested that you use Gel-coated microscopic slides for tissue mounting and 0.17um-thick coverslips.
• A 15% of the fee will be due upon authorization of the study; and the remaining fee will be due upon delivery of study results.
• Progress of the service is contingent upon staining quality of tissues, operated by the independent contractor.
• Should early termination occur, Neurodigitech will prorate the cost incurred and invoice the Sponsor. The first portion of the fee is non-refundable.