Abridged Genetic Engineering Sequence

THE BIG PICTURE

STUDENT GUIDE FOR ABRIDGED GENETIC ENGINEERING SEQUENCE

PRINT MANUAL PAGES 7-16

PDF PAGES 7-18

Treating Disease with Gene Cloning

Dateline: America "Teenage Diabetes on the Rise"

Diabetes Types 1 and 2

Your Challenge

PRINT MANUAL PAGES 17-25

PDF PAGES 19-27

How to use the Micropipette

Introduction: p 19

Lab: p 20-25

PRINT MANUAL PAGES 26-34

PDF PAGES 28-36

Part A: Pipetting in the Wells

Lab: p 26-29

Part B: Separating Dyes with Gel Electrophoresis

Lab: p 29-34

PRINT MANUAL PAGES 37-53

PDF PAGES 37-54

Preparing to verify the RFP gene: Digesting the pARA-R plasmid

Introduction: p 37-45

Lab: p 46-53

PRINT MANUAL PAGES 57-68

PDF PAGES 57-70

Verification of the Recombinant Plasmid using Gel Electrophoresis

Introduction: p 57-62

Lab: p 63-68

PRINT MANUAL PAGES 71-86

PDF PAGES 71-88

Transforming Bacteria with the pARA-R Plasmid

Introduction: p 71-75

Lab: p 76-86