Tenders are invited in the department

Post date: Mar 22, 2018 8:9:29 AM

No. DCB/SIUCEB/423/17-18 22/03/2018

Tender Notice

Sub :Tenders invited for Next Generation Sequencing (NGS) service

Competitive Tenders are invited from service providers for carrying out Next Generation Sequencing

work as shown in Annexure. Tenders should specifically show the charges, taxes, forwarding charges and

terms of delivery separately. Evaluation will be based on total amount

Validity of the offer and the time required for effecting the sequencing work after placing the order should

also be mentioned in the Tender.

Successful bidder has to complete the work within the stipulated time failing which the order will be

treated as cancelled without any further intimation. You are requested to quote the reference number and

date of this letter in all your correspondence.

Terms and Conditions

1. Each bidder shall submit only one Tender

2. Fax/email bids shall not be accepted.

3. Late/Delayed and tenders shall not be opened.

4. Wrongly referenced / enclosed Tenders and Unsigned (wherever signature required) bids will be

rejected summarily

5. The companies participating should provide the Installation certificate for PacBio in their facility in

India

6. The cost should be mentioned separately for sequencing using different platforms and

bioinformatics analysis

7. Payment conditions: 100% payment on receipt of the results as per specifications.

8. The sample should not be sent outside India for sequencing.

9. Delivery: To be made available to this Institute in soft copies and hard disc.

Annexure

Tenders are invited from firms providing RNA sequencing using next generation sequencing platforms for

undertaking the RNA sequencing and bioinformatics analysis of the Trichopus zeylanicus using Illumina

Hiseq 2500

The details of the plant species and sequencing specifications are given below.

Details of the plant species

Common name - Arogyapacha

Scientific Name - Trichopus zeylanicus

Chromosome number - 2n= 28

Genome size (haploid) - ~ 1 Gb

Specifications for sequencing

1. RNA Sequencing and analysis of Trichopus zeylanicus Using Illumina HiSeq 2500 platform.

2. De Novo Transcriptome Sequencing, assembly and Bioinformatic Analysis of Trichopus

zeylanicus samples on Illumina HiSeq 2500/4000 Platform for 4 samples.

3. Primary Bioinformatics analysis should include Base Calling, Filtering, QC, Trimming,

contamination removal.

4. Secondary Bioinformatics analysis should include Error correction, Denovo Assembly and

Mapping of Transcriptome to the Hybrid assembled Genome.

The following information also should be provided in the Tender.

a. Proposed sequencing and assembly strategies in detail for Trichopus zeylanicus

b. Type of sequencing platform (s) including software/ programme modules to be used.

c. Modal read lengths for different platforms

d. Depth of sequencing

e. Details of all bioinformatics services to be provided

All interested service providers are requested to send their Tender in sealed envelope superscribing

“Tender No. DCB/SIUCEB/423/17-18–dt 22/03/2018” for Next Generation Sequencing Work” to “The

Director, SIUCEB, Department of Computational Biology and Bioinformatics, University of Kerala,

Kariavattom, Trivandrum, Kerala, India- 695581” on or before 5pm on 06-04- 2018. The received

Tenders will be opened on the next working day at 10am.

Director, SIUCEB

Department of Computational Biology and Bioinformatics