Quotations invited in the Department

Post date: Feb 28, 2020 11:18:53 AM

No. /DCB/439/2019-2020 27/02/2020

NOTIFICATION

Sub: Tenders invited for Next Generation Sequencing (NGS) service

Competitive Tenders are invited from service providers for carrying out Metagenome sequencing work as shown in Annexure. Tenders should specifically show the charges, taxes, forwarding charges and terms of delivery separately. Evaluation will be based on total amount

Validity of the offer and the time required for effecting the sequencing work after placing the order should also be mentioned in the Tender.

Successful bidder has to complete the work within the stipulated time failing which the order will be treated as cancelled without any further intimation. You are requested to quote the reference number and date of this letter in all your correspondence.

Terms and Conditions

• 1. Each bidder shall submit only one Tender

  2. Fax/email bids shall not be accepted.

  3. Late/Delayed and tenders shall not be opened.

  4. Wrongly referenced / enclosed Tenders and Unsigned (wherever signature required) bids will be rejected summarily

  5. The cost should be mentioned separately for sequencing using different platforms and bioinformatics analysis

  6. Payment conditions: 100% payment on receipt of the results as per specifications.

  7. The sample should not be sent outside India for sequencing.

  8. Delivery: To be made available to this Institute in soft copies and hard disc.

Annexure

Tenders are invited from firms providing DNA sequencing services with proven track record of whole genome sequencing and de novo assembly for undertaking the Metagenome sequencing (16s rRNA) of human gut microbiome (2 samples).

Specifications for sequencing

• 1. Metagenome Sequencing and analysis of human gut microbiome samples using Illumina HiSeqX platforms

  2. Paired-end sequencing 2x150bp sequencing.

  3. Primary Bioinformatics analysis should include Base Calling, Filtering, Trimming

  4. Secondary Bioinformatics analysis should include Error correction and count data, OTU classification and cladogram, OTU abundance

  5. The final report should contain Statistics of raw reads, Raw data QC, Statistics of clean and filtered reads, Statistics after error correction, OTU statistics, OTU cladogram

All interested service providers are requested to send their Tender in sealed envelope super scribing “Tender No. DCB/ADMIN/439/2019-2020–dt 27/02/2020” for Metagnome sequencing” to “The Head, Department of Computational Biology and Bioinformatics, University of Kerala, Kariavattom, Trivandrum, Kerala, India- 695581” on or before 5pm on 16-03-2020. The received Tenders will be opened on the next working day at 10am.