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The Agilent HPLC 1200 is an advanced high-performance liquid chromatography system used for complex analytical testing in pharmacy laboratories at Monash University Malaysia. This precise instrument utilises a quaternary solvent delivery system to allow for accurate gradient separations, an autosampler for automated sample injection, and a diode array detector and fluorescence detector to identify compounds by UV absorption and fluorescence, respectively. The system is also equipped with a sample collector and powerful software that allows for full control and optimisation of HPLC parameters for techniques like method development and quality control of drug formulations. Though configured at the analytical scale, the HPLC 1200 offers exceptional flexibility, sensitivity, and reliability, enabling students and researchers to perform cutting-edge pharmaceutical analysis on small molecules, proteins, and peptides. This high-throughput workhorse drives innovation in drug discovery and development at Monash Malaysia.