Binghamton Research Days Student Presentations
Direct Incorporation of Fluorescent Non-canonical Amino Acid L-ANAP into HEK-293 Cells to Label β-Tubulin.
Direct Incorporation of Fluorescent Non-canonical Amino Acid L-ANAP into HEK-293 Cells to Label β-Tubulin.
Linda Cheung (Sophomore, Biochemistry); Zirong Chen (Sophomore, Biochemistry); Ryan Hoskins (Sophomore, Biochemistry); James Maine (Sophomore, Biology and French); Benjamin Weisman (Sophomore, Integrative Neuroscience)
Linda Cheung (Sophomore, Biochemistry); Zirong Chen (Sophomore, Biochemistry); Ryan Hoskins (Sophomore, Biochemistry); James Maine (Sophomore, Biology and French); Benjamin Weisman (Sophomore, Integrative Neuroscience)
Mentor: Susan Flynn, Chemistry
Mentor: Susan Flynn, Chemistry
Abstract
For decades, fluorescent proteins (FPs) have been used to label proteins, allowing for the monitoring of protein behavior and localization. However, FPs are relatively large in size, and have the potential to disturb intimate protein-protein interactions and pathways. This issue can be potentially alleviated with the application of a novel technique; fluorescent non-canonical amino acids (ncAAs) directly incorporated into proteins of interest. Here we explore this newer approach to labeling proteins with fluorescent ncAA 3-(6-acetylnaphthalen-2ylamino)-2- aminopropanoic acid (Anap). For an alternative method to observe microtubule dynamics, Anap can be successfully incorporated into β-tubulin in mammalian systems.
Abstract
For decades, fluorescent proteins (FPs) have been used to label proteins, allowing for the monitoring of protein behavior and localization. However, FPs are relatively large in size, and have the potential to disturb intimate protein-protein interactions and pathways. This issue can be potentially alleviated with the application of a novel technique; fluorescent non-canonical amino acids (ncAAs) directly incorporated into proteins of interest. Here we explore this newer approach to labeling proteins with fluorescent ncAA 3-(6-acetylnaphthalen-2ylamino)-2- aminopropanoic acid (Anap). For an alternative method to observe microtubule dynamics, Anap can be successfully incorporated into β-tubulin in mammalian systems.